Correction to: A Versatile Vector Toolkit for Functional Analysis of Rice Genes.

The caption of Fig. 5 and the figure contained an error [1]. The updated figure and caption are published in this correction article.

Correction

The caption of Fig. 5 contained an error. The updated caption along with the original figure is published in this correction article.

Fig. 5

Diagram of how CRISPR/Cas9 vectors were constructed and used to edit the rice IPA1 gene. a Western blot analysis of the Cas9-intron expression in rice protoplasts. HSP indicates the loading amount of each sample. b Target site selection for candidate genes in the rice genome. A 20-bp specific sequence followed by the PAM “NGG” structure is required. c and d Target cloning to the entry vectors. Synthesis of the primer pairs of the 20-bp specific target with the 4-bp adapters, and ligation with the BsaI linearized pEntry A or pEntry B vector. e One-step ligation and f step-by-step ligation of multiple targets to pRHCas9/pRGCas9. Four pairs of isocaudamers, PstI(E1)-NsiI(E1'), XbaI(E2)-SpeI(E2'), BamHI(E3)-BglII(E3'), and SalI(E4)-XhoI(E4') are marked. The sgRNA cassettes with U6P1 and U6P2 in pEntry A and B should be used in turn. g Representative sequencing chromatogram of the CRISPR-IPA1 transgenic lines. Line #7, wild-type genotype; line #5, mutant genotype. h Representative gene editing results in the CRISPR-IPA1 transgenic lines

Table S1 in the Additional file of this original publication contained some errors. The updated Table S1 is published in this correction article.

Updated Table S1, the full supplementary materials can be downloaded from the original publication. (XLSX 10 kb)