Hyojin Kim1, Jeong Mi Yang1, Soon-Hyun Ahn2, Woo-Jin Jeong3, Jin-Haeng Chung1, Jin Ho Paik4. 1. Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. 2. Department of Otorhinolaryngology, Seoul National University Hospital, Seoul, Republic of Korea. 3. Department of Otorhinolaryngology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. 4. Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea paikjh@snu.ac.kr.
Abstract
BACKGROUND: Altered microRNA expression is associated with cancer progression. This study investigated the prognostic significance of microRNA-155-5p (miR-155-5p), a well-known oncomiR, in oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: miR-155-5p expression was assessed using quantitative reverse-transcription polymerase chain reaction in 68 formalin-fixed, paraffin-embedded OSCC specimens. E-Cadherin immunohistochemistry was conducted to correlate epithelial-mesenchymal transition (EMT) with miR-155-5p expression. RESULTS: Elevated miR-155-5p was associated with higher pathological TNM stage (p=0.048) and relapse (p=0.029). High miR-155-5p expression, along with angiolymphatic invasion and advanced stage, was a statistically significant prognostic factor for poorer disease-free survival. In patients with stage I-II disease, high miR-155-5p was the only significant prognostic factor (p=0.033). A significant negative correlation was observed between miR-155-5p and E-cadherin expression (p=0.015), suggesting a possible role for miR-155-5p in EMT. CONCLUSION: miR-155-5p expression might contribute to EMT-associated OSCC progression and serve as a biomarker for predicting relapse, especially for patients with early-stage OSCC. Copyright
BACKGROUND: Altered microRNA expression is associated with cancer progression. This study investigated the prognostic significance of microRNA-155-5p (miR-155-5p), a well-known oncomiR, in oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS:miR-155-5p expression was assessed using quantitative reverse-transcription polymerase chain reaction in 68 formalin-fixed, paraffin-embedded OSCC specimens. E-Cadherin immunohistochemistry was conducted to correlate epithelial-mesenchymal transition (EMT) with miR-155-5p expression. RESULTS: Elevated miR-155-5p was associated with higher pathological TNM stage (p=0.048) and relapse (p=0.029). High miR-155-5p expression, along with angiolymphatic invasion and advanced stage, was a statistically significant prognostic factor for poorer disease-free survival. In patients with stage I-II disease, high miR-155-5p was the only significant prognostic factor (p=0.033). A significant negative correlation was observed between miR-155-5p and E-cadherin expression (p=0.015), suggesting a possible role for miR-155-5p in EMT. CONCLUSION:miR-155-5p expression might contribute to EMT-associated OSCC progression and serve as a biomarker for predicting relapse, especially for patients with early-stage OSCC. Copyright
Authors: Mario Dioguardi; Francesca Spirito; Diego Sovereto; Lucia La Femina; Alessandra Campobasso; Angela Pia Cazzolla; Michele Di Cosola; Khrystyna Zhurakivska; Stefania Cantore; Andrea Ballini; Lorenzo Lo Muzio; Giuseppe Troiano Journal: Biology (Basel) Date: 2022-04-24