Catherine A Wilhelm1, Zackary J Clor1, Jessica L Kelts2. 1. Department of Chemistry and Biochemistry, University of Michigan-Flint, Flint, MI, U.S.A. 2. Department of Chemistry and Biochemistry, University of Michigan-Flint, Flint, MI, U.S.A. jkelts@umflint.edu.
Abstract
BACKGROUND/AIM: Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer that disproportionately affects women with darker skin and is often treated with paclitaxel (PTX). Here, the effect of vitamin D on p53-positive DU4475 cells and its ability to decrease the IC50 of PTX in these cells were investigated. MATERIALS AND METHODS: The growth inhibitory effects of vitamin D on DU4475 cells and the effect of PTX plus vitamin D on overall TNBC cell viability was assessed using CellTiter-Glo®. RESULTS: Vitamin D increased proliferation of DU4475 cells at low concentrations and lowered the IC50 of PTX. However, it did not change the IC50 of PTX in MDA-MB-231 cells which remained largely viable. CONCLUSION: The effect of vitamin D on DU4475 cell viability was different than in other TNBC cells. The effect of PTX on DU4475 cells was enhanced with vitamin D. MDA-MB-231 cells were relatively resistant to the effects of PTX. Copyright
BACKGROUND/AIM: Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer that disproportionately affects women with darker skin and is often treated with paclitaxel (PTX). Here, the effect of vitamin D on p53-positive DU4475 cells and its ability to decrease the IC50 of PTX in these cells were investigated. MATERIALS AND METHODS: The growth inhibitory effects of vitamin D on DU4475 cells and the effect of PTX plus vitamin D on overall TNBC cell viability was assessed using CellTiter-Glo®. RESULTS:Vitamin D increased proliferation of DU4475 cells at low concentrations and lowered the IC50 of PTX. However, it did not change the IC50 of PTX in MDA-MB-231 cells which remained largely viable. CONCLUSION: The effect of vitamin D on DU4475 cell viability was different than in other TNBC cells. The effect of PTX on DU4475 cells was enhanced with vitamin D. MDA-MB-231 cells were relatively resistant to the effects of PTX. Copyright
Authors: Nadeem Bilani; Leah Elson; Charles Szuchan; Elizabeth Elimimian; Mustafa Saleh; Zeina Nahleh Journal: In Vivo Date: 2021-04-28 Impact factor: 2.406