Dhanalakshmi Ravikumar1, Mahesh R1, Sharna Ningthoujam2, Waikhom Robindro3, Gayathri R4, Vishnu Priya V4. 1. PhD Scholar, Department of Pedodontics and Preventive Dentistry, Saveetha Dental College, Chennai. 2. Dr.Ningthoujam sharna- Private Practice, Manipur, Imphal, India. 3. Department of Oral and Maxillofacial Surgery, RIMS, Imphal, India. 4. Department of Biochemistry, Saveetha Dental College, Chennai, Tamil Nadu, India.
Abstract
OBJECTIVES: At present, there is lack of evidence in literature regarding the transmission of Streptococcus Mutans from a caries-free mother to child by genetic methods, so the present study aimed to identify the genetic characterization of Streptococcus Mutans strains isolated from caries-free and caries active children and their mother being caries-free in both the groups and also to identify the genetic patterns of Streptococcus Mutans between caries-free and caries-active individuals. METHODS: Twenty child-mother pair were selected and divided into 2 groups and the mothers being caries-free in both the groups. Saliva samples were collected using a sterile tube, followed by microbial culture of Streptococcus mutans, DNA isolation and PCR amplification. The molecular weights of each band were converted into a binary data and data were entered into SPSS software version 20.0 to generate similarity dendrograms. RESULTS: Amplified products of Streptococcus Mutans demonstrated a same genetic distance between the mother-child pair, indicative of a closely related species. Dendrogram interpretation represented a greater genetic polymorphism of Streptococcus Mutans between caries-free and caries-active children. CONCLUSIONS: Identical genetic distance between child-mother pair showed that, these Streptooccus Mutans were closely related and could have vertically transmitted from their mothers. Different genotypic pattern between caries-free and caries active subjects showed a genetic polymorphism among the Streptococcus Mutans strains.
OBJECTIVES: At present, there is lack of evidence in literature regarding the transmission of Streptococcus Mutans from a caries-free mother to child by genetic methods, so the present study aimed to identify the genetic characterization of Streptococcus Mutans strains isolated from caries-free and caries active children and their mother being caries-free in both the groups and also to identify the genetic patterns of Streptococcus Mutans between caries-free and caries-active individuals. METHODS: Twenty child-mother pair were selected and divided into 2 groups and the mothers being caries-free in both the groups. Saliva samples were collected using a sterile tube, followed by microbial culture of Streptococcus mutans, DNA isolation and PCR amplification. The molecular weights of each band were converted into a binary data and data were entered into SPSS software version 20.0 to generate similarity dendrograms. RESULTS: Amplified products of Streptococcus Mutans demonstrated a same genetic distance between the mother-child pair, indicative of a closely related species. Dendrogram interpretation represented a greater genetic polymorphism of Streptococcus Mutans between caries-free and caries-active children. CONCLUSIONS: Identical genetic distance between child-mother pair showed that, these Streptooccus Mutans were closely related and could have vertically transmitted from their mothers. Different genotypic pattern between caries-free and caries active subjects showed a genetic polymorphism among the Streptococcus Mutans strains.
Authors: Mitzi R Becker; Bruce J Paster; Eugene J Leys; Melvin L Moeschberger; Sarah G Kenyon; Jamie L Galvin; Susan K Boches; Floyd E Dewhirst; Ann L Griffen Journal: J Clin Microbiol Date: 2002-03 Impact factor: 5.948
Authors: Karolina Gerreth; Timucin Ari; Wojciech Bednarz; Michal Nowicki; Maria Borysewicz-Lewicka Journal: Med Princ Pract Date: 2019-09-16 Impact factor: 1.927