J Marc Rhoads1, James Collins2, Nicole Y Fatheree3, S Shahrukh Hashmi4, Christopher M Taylor5, Meng Luo5, Thomas K Hoang3, Wallace A Gleason3, Melissa R Van Arsdall3, Fernando Navarro3, Yuying Liu3. 1. Department of Pediatrics, Gastroenterology Division, McGovern Medical School, UTHealth, Houston, TX. Electronic address: j.marc.rhoads@uth.tmc.edu. 2. Department of Molecular Virology and Microbiology, Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, TX. 3. Department of Pediatrics, Gastroenterology Division, McGovern Medical School, UTHealth, Houston, TX. 4. Department of Pediatrics, Pediatric Research Center, McGovern Medical School, UTHealth, Houston, TX. 5. Department of Microbiology, Immunology & Parasitology, LSU Health Sciences Center New Orleans, New Orleans, LA.
Abstract
OBJECTIVE: To dissect potential confounding effects of breast milk and formula feeding on crying + fussing, fecal calprotectin, and gut microbiota in babies with colic. We hypothesized that infant colic is associated with gut inflammation linked to intestinal dysbiosis. STUDY DESIGN: A nested case-control design of 3 of our studies was used to analyze clinical and laboratory data at presentation, comparing babies with colic with controls. All investigators other than the biostatistician were blinded during data analysis. Subjects were recruited based on their age and crying + fussy time. We screened 65 infants, 37 with colic, as defined by Barr diary (crying + fussing time >3 hours daily), who were compared with 28 noncolicky infants. RESULTS: Fecal calprotectin was elevated in babies with colic. For each mode of infant feeding (breast milk, formula, or breast + formula), infants' fecal calprotectin was higher in babies with colic. Infants with colic had similar levels of fecal alpha diversity (richness) when compared with controls, and alpha diversity was lower in breast-fed babies. Beta diversity at the phylum level revealed significant differences in microbial population. A phylum difference resulted from reduced Actinobacteria (95% of which are Bifidobacilli) in babies with colic. Species significantly associated with colic were Acinetobacter and Lactobacillus iners. CONCLUSIONS: Colic is linked with gut inflammation (as determined by fecal calprotectin) and dysbiosis, independent of mode of feeding, with fewer Bifidobacilli. TRIAL REGISTRATION: Clinicaltrials.gov: NCT01279265 and NCT01849991. Published by Elsevier Inc.
OBJECTIVE: To dissect potential confounding effects of breast milk and formula feeding on crying + fussing, fecal calprotectin, and gut microbiota in babies with colic. We hypothesized that infant colic is associated with gut inflammation linked to intestinal dysbiosis. STUDY DESIGN: A nested case-control design of 3 of our studies was used to analyze clinical and laboratory data at presentation, comparing babies with colic with controls. All investigators other than the biostatistician were blinded during data analysis. Subjects were recruited based on their age and crying + fussy time. We screened 65 infants, 37 with colic, as defined by Barr diary (crying + fussing time >3 hours daily), who were compared with 28 noncolicky infants. RESULTS: Fecal calprotectin was elevated in babies with colic. For each mode of infant feeding (breast milk, formula, or breast + formula), infants' fecal calprotectin was higher in babies with colic. Infants with colic had similar levels of fecal alpha diversity (richness) when compared with controls, and alpha diversity was lower in breast-fed babies. Beta diversity at the phylum level revealed significant differences in microbial population. A phylum difference resulted from reduced Actinobacteria (95% of which are Bifidobacilli) in babies with colic. Species significantly associated with colic were Acinetobacter and Lactobacillus iners. CONCLUSIONS: Colic is linked with gut inflammation (as determined by fecal calprotectin) and dysbiosis, independent of mode of feeding, with fewer Bifidobacilli. TRIAL REGISTRATION: Clinicaltrials.gov: NCT01279265 and NCT01849991. Published by Elsevier Inc.
Authors: Julie D Thai; Sara Cherkerzian; Evgenia J Filatava; Ngan Luu; Hidemi S Yamamoto; Raina N Fichorova; Mandy B Belfort; Katherine E Gregory Journal: J Pediatr Gastroenterol Nutr Date: 2022-04-26 Impact factor: 3.288
Authors: Troy A Markel; Colin A Martin; Hala Chaaban; Jennifer Canvasser; Heather Tanner; Heather Denchik; Misty Good Journal: Pediatr Res Date: 2020-08 Impact factor: 3.756
Authors: Yuying Liu; Thomas K Hoang; Christopher M Taylor; Evelyn S Park; Jasmin Freeborn; Meng Luo; Stefan Roos; J Marc Rhoads Journal: Am J Physiol Gastrointest Liver Physiol Date: 2021-03-31 Impact factor: 4.871