Chandrakala Nagarajappa1, Sheela Shikaripur Rangappa2, Ravishankar Suryanarayana3, Sharath Balakrishna4. 1. Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, India. 2. Department of Obstetrics and Gynecology, Sri Devaraj Urs Medical College, Kolar, India. 3. Department of Community Medicine, Sri Devaraj Urs Medical College, Kolar, India. 4. Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, India. Electronic address: sharath@sduu.ac.in.
Abstract
OBJECTIVES: To evaluate the presence of urinary congophilia amongIndian patients with preeclampsia. STUDY DESIGN: A prospective case control study in which congophilia of urine samples from preeclamptic pregnant women (n = 62) and normotensive pregnant women (n = 65) was compared by using Congo Red Dot Blot assay. MAIN OUTCOME MEASURES: Presence of urinary congophilia. RESULTS: Mean percentage of Congo Red Retention was 37.9 ± 4.1 in the normotensive pregnant group and 77.9 ± 11.5 in the preeclamptic pregnant group (P < .001). The mean percentage of Congo Red Retention in both early-onset (70.5 ± 9.0) and late-onset (82.7 ± 10.3) groups were significantly higher than in normotensive controls (P < .001). The mean percentage of Congo Red Retention in mild (61.2 ± 3.2) and severe (82.4 ± 8.4) types of preeclampsia were also as significantly higher than in normotensive controls (P < .001). The mean percentage of Congo Red Retention in preeclampsia superimposed by eclampsia (89.4 ± 2.0) and preeclampsia complicated by intrauterine growth restriction and intrauterine death (74.6 ± 5.8) were significantly higher than in normotensive controls (P < .001). CONCLUSIONS: The results of this study confirms the presence of urinary congophilia in Indian pregnant women with preeclampsia. Furthermore, our study shows that urinary congophilia is not affected by clinical variables like gestational age of onset, severity, superimposition by eclampsia and complication by intrauterine growth restriction and intrauterine death. Urinary congophilia can be used to differentially identify preeclamptic pregnant women from normotensive pregnant women.
OBJECTIVES: To evaluate the presence of urinary congophilia amongIndian patients with preeclampsia. STUDY DESIGN: A prospective case control study in which congophilia of urine samples from preeclamptic pregnant women (n = 62) and normotensive pregnant women (n = 65) was compared by using Congo Red Dot Blot assay. MAIN OUTCOME MEASURES: Presence of urinary congophilia. RESULTS: Mean percentage of Congo Red Retention was 37.9 ± 4.1 in the normotensive pregnant group and 77.9 ± 11.5 in the preeclamptic pregnant group (P < .001). The mean percentage of Congo Red Retention in both early-onset (70.5 ± 9.0) and late-onset (82.7 ± 10.3) groups were significantly higher than in normotensive controls (P < .001). The mean percentage of Congo Red Retention in mild (61.2 ± 3.2) and severe (82.4 ± 8.4) types of preeclampsia were also as significantly higher than in normotensive controls (P < .001). The mean percentage of Congo Red Retention in preeclampsia superimposed by eclampsia (89.4 ± 2.0) and preeclampsia complicated by intrauterine growth restriction and intrauterine death (74.6 ± 5.8) were significantly higher than in normotensive controls (P < .001). CONCLUSIONS: The results of this study confirms the presence of urinary congophilia in Indian pregnant women with preeclampsia. Furthermore, our study shows that urinary congophilia is not affected by clinical variables like gestational age of onset, severity, superimposition by eclampsia and complication by intrauterine growth restriction and intrauterine death. Urinary congophilia can be used to differentially identify preeclamptic pregnant women from normotensive pregnant women.
Authors: Kara M Rood; Catalin S Buhimschi; Theresa Dible; Shaylyn Webster; Guomao Zhao; Philip Samuels; Irina A Buhimschi Journal: EClinicalMedicine Date: 2019-03-01