| Literature DB >> 30175994 |
Weiyue Zhang1, Wei Zhang1, Ning Dai1, Chenxia Han2, Fengzhi Wu1, Xu Wang1, Libo Tan1, Jie Li1, Feng Li3, Qingjia Ren4.
Abstract
In this article, we introduced a rat model of central fatigue using the modified multiple platform method (MMPM). The Multiple Platform box was designed as a water tank with narrow platforms on the bottom. The model rats were put into the tank and stood on the platforms for 14 h (18:00 - 8:00) per day for a consecutive 21 days, with a blank control group set for contrast. At the end of modeling, rats in the model group showed an obvious fatigued appearance. To assess the model, we performed several behavioral tests, including the open field test (OFT), the elevated plus maze (EPM) test, and the exhaustive swimming (ES) test. The results showed that anxiety, spatial cognition impairment, poor muscle performance, and declined voluntary activity presented in model rats confirm the diagnosis of central fatigue. Changes of the central neurotransmitters also verified the result. In conclusion, the model successfully simulated central fatigue, and future study with the model may help reveal the pathological mechanism of the disease.Entities:
Mesh：
Year: 2018 PMID： 30175994 PMCID： PMC6126791 DOI： 10.3791/57362
Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355