Luiza Lucy Oliveira Rodrigues1, Ana Carolina Leal de Oliveira2, Shams Tabrez3, Shazi Shakil4, Mohammad Imran Khan5, Muhammad Nadeem Asghar6, Bianca Dias Matias7, Joysa Michelle Alves da Silva Batista8, Marinilva Modesto Rosal9, Marylia Maria Duarte Fulgencio de Lima10, Sávia Ribeiro Ferreira Gomes11, Rodrigo Mendes de Carvalho12, Germano Pinho de Moraes13, Marcus Vinicius Oliveira Barros de Alencar14, Muhammad Torequl Islam15, Ana Amélia de Carvalho Melo-Cavalcante16. 1. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: luizalucy@hotmail.com. 2. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: carol_leal00@hotmail.com. 3. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: shamstabrez1@gmail.com. 4. Center of Innovation in Personalized Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: shazibiotech@gmail.com. 5. Department of Biochemistry, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: mikhan@kau.edu.sa. 6. Department of Medical Biology, University of Quebec, Trois Riveres, Canada. Electronic address: muhammad.nadeem.asghar2@ugtr.ca. 7. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: bianca_matias@hotmail.com. 8. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: joysa_michelle@hotmail.com. 9. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: marinilvamodesto@gmail.com. 10. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: marymama_duarte@hotmail.com. 11. Graduate Program, Faculty of Medicine, NOVAFAPI, Teresina, PI, Brazil. Electronic address: saviagomes@hotmail.com. 12. Graduate Program, Faculty of Biochemistry, NOVAFAPI, Teresina, PI, Brazil. Electronic address: rodrigomendesbio78@gmail.com. 13. Postgraduate Program in Cellular Biology Applied to Health (PPGBioSaúde), Lutheran University of Brazil, Canoas, Brazil. Electronic address: germanopinho@uol.com.br. 14. Post-Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, PI, Brazil. Electronic address: marcus-alencar@msn.com. 15. Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam; Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam. Electronic address: muhammad.torequl.islam@tdt.edu.vn. 16. Post-Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, PI, Brazil. Electronic address: anameliamelocavalcante@gmail.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Aloe vera is a widely used medicinal plant for its various biological activities. This study evaluate possible mutagenic and healing effects of the aqueous extract of A. vera (AEAV) in mice and its oxidant/antioxidant potential in different proficient and deficient Saccharomyces cerevisiae strains. MATERIAL AND METHODS: The AEAV was topically treated on the wounded skin surface of male albino mice at doses of 10 and 50 mg/kg for seven successive days. The control group was similarly treated with 0.9% NaCl solution. For oxidative/anti-oxidative evaluation, both proficient and deficient strains of S. cerevisiae [cytoplasmic and mitochondrial superoxide dismutase mutant (SOD: Sod1Δ and Sod2Δ), cytoplasmic catalase mutant (CAT: Cat1Δ)], two double defective mutants of Sod1 and Sod2 and Sod1 and Cat1 genes along with a wild-type strains were used. RESULTS: The healing property of AEAV was observed at the dose of 50 mg/kg but at the same dose it showed mutagenic and cytotoxic effects in peripheral blood. AEAV did not produce the oxidizing effect, except in the mutated CAT strain at highest concentration (50 mg/kg). CONCLUSION: The high concentration of AEAV showed mutagenicity and cytotoxicity. Beside, the healing capacity is believed to be due to its anti-oxidative defense mechanism.
ETHNOPHARMACOLOGICAL RELEVANCE: Aloe vera is a widely used medicinal plant for its various biological activities. This study evaluate possible mutagenic and healing effects of the aqueous extract of A. vera (AEAV) in mice and its oxidant/antioxidant potential in different proficient and deficient Saccharomyces cerevisiae strains. MATERIAL AND METHODS: The AEAV was topically treated on the wounded skin surface of male albino mice at doses of 10 and 50 mg/kg for seven successive days. The control group was similarly treated with 0.9% NaCl solution. For oxidative/anti-oxidative evaluation, both proficient and deficient strains of S. cerevisiae [cytoplasmic and mitochondrial superoxide dismutase mutant (SOD: Sod1Δ and Sod2Δ), cytoplasmic catalase mutant (CAT: Cat1Δ)], two double defective mutants of Sod1 and Sod2 and Sod1 and Cat1 genes along with a wild-type strains were used. RESULTS: The healing property of AEAV was observed at the dose of 50 mg/kg but at the same dose it showed mutagenic and cytotoxic effects in peripheral blood. AEAV did not produce the oxidizing effect, except in the mutated CAT strain at highest concentration (50 mg/kg). CONCLUSION: The high concentration of AEAV showed mutagenicity and cytotoxicity. Beside, the healing capacity is believed to be due to its anti-oxidative defense mechanism.
Authors: Stefania Vitale; Sara Colanero; Martina Placidi; Giovanna Di Emidio; Carla Tatone; Fernanda Amicarelli; Anna Maria D'Alessandro Journal: Molecules Date: 2022-06-01 Impact factor: 4.927
Authors: Dipanjan Karati; Ryan Varghese; K R Mahadik; Rohit Sharma; Dileep Kumar Journal: Evid Based Complement Alternat Med Date: 2022-07-20 Impact factor: 2.650