Ivson Cassiano de Oliveira Santos1, Rodolpho Mattos Albano2, Marise Dutra Asensi1, Ana Paula D'Alincourt Carvalho-Assef3. 1. Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil. 2. Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. 3. Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil. Electronic address: anapdca@ioc.fiocruz.br.
Abstract
OBJECTIVES: Klebsiella pneumoniae carbapenemase (KPC) is the most widespread carbapenemase in Enterobacteriaceae in Brazil. Although its presence is not common in Pseudomonas aeruginosa, it has been increasingly reported. Here we report a draft genome sequence of a KPC-producing P. aeruginosa strain recovered from a bloodstream infection sample in Brazil. METHODS: The antimicrobial susceptibility of KPC-producing P. aeruginosa CCBH17348 was evaluated by the disk diffusion method, Etest and broth microdilution. Carbapenemase production was confirmed by colorimetric assay (Carba NP) and PCR. Genomic DNA was sequenced by Illumina MiSeq sequencing and was assembled using the A5-Miseq pipeline, and gene annotation was performed using RAST 2.0. The database ResFinder 2.1, CRISPRFinder and MLST website were used to identify resistance genes, clustered regularly interspaced short palindromic repeats (CRISPRs) and sequence types (STs), respectively. RESULTS: Isolate CCBH17348 was considered multidrug-resistant, was susceptible to fluoroquinolones, gentamicin and polymyxin, and belonged to a newly described ST (ST2584), carrying an IncQ1 plasmid with blaKPC-2 and aph(3')-VI genes. Other genes associated with resistance and virulence found in the genome were blaOXA-50, blaPAO, fosA, catB, mutation in oprD and mexT (MexEF-OprN efflux regulator), and exotoxin-encoding genes (exoS, exoY and exoT). CONCLUSIONS: This study highlights the potential risk of new STs of P. aeruginosa carrying blaKPC-2 and the potential spread of blaKPC-2 in an IncQ1 plasmid.
OBJECTIVES:Klebsiella pneumoniae carbapenemase (KPC) is the most widespread carbapenemase in Enterobacteriaceae in Brazil. Although its presence is not common in Pseudomonas aeruginosa, it has been increasingly reported. Here we report a draft genome sequence of a KPC-producing P. aeruginosa strain recovered from a bloodstream infection sample in Brazil. METHODS: The antimicrobial susceptibility of KPC-producing P. aeruginosa CCBH17348 was evaluated by the disk diffusion method, Etest and broth microdilution. Carbapenemase production was confirmed by colorimetric assay (Carba NP) and PCR. Genomic DNA was sequenced by Illumina MiSeq sequencing and was assembled using the A5-Miseq pipeline, and gene annotation was performed using RAST 2.0. The database ResFinder 2.1, CRISPRFinder and MLST website were used to identify resistance genes, clustered regularly interspaced short palindromic repeats (CRISPRs) and sequence types (STs), respectively. RESULTS: Isolate CCBH17348 was considered multidrug-resistant, was susceptible to fluoroquinolones, gentamicin and polymyxin, and belonged to a newly described ST (ST2584), carrying an IncQ1 plasmid with blaKPC-2 and aph(3')-VI genes. Other genes associated with resistance and virulence found in the genome were blaOXA-50, blaPAO, fosA, catB, mutation in oprD and mexT (MexEF-OprN efflux regulator), and exotoxin-encoding genes (exoS, exoY and exoT). CONCLUSIONS: This study highlights the potential risk of new STs of P. aeruginosa carrying blaKPC-2 and the potential spread of blaKPC-2 in an IncQ1 plasmid.
Authors: Mila M Almeida; Meyvianne T Freitas; Tania W Folescu; Monica C Firmida; Ana Paula D'A Carvalho-Assef; Elizabeth A Marques; Robson S Leão Journal: Curr Microbiol Date: 2021-01-06 Impact factor: 2.188
Authors: Elisa Maria Beirão; Suellen da Silva Rodrigues; Tarik Klain de Andrade; Fernando Brandão Serra; Marina Della Negra de Paula; Thales Jose Bueno Polis; Ana Cristina Gales Journal: Braz J Infect Dis Date: 2020-07-12 Impact factor: 3.257
Authors: Willames M B S Martins; Marisa F Nicolas; Yang Yu; Mei Li; Priscila Dantas; Kirsty Sands; Edward Portal; Luiz G P Almeida; Ana Tereza R Vasconcelos; Eduardo A Medeiros; Mark A Toleman; Timothy R Walsh; Ana C Gales; Diego O Andrey Journal: mSphere Date: 2020-10-07 Impact factor: 4.389