Comparative analysis of mitochondrial genomes of the superfamily Grylloidea (Insecta, Orthoptera) reveals phylogenetic distribution of gene rearrangements.

To further characterize mitochondrial genome (mitogenome) features of the superfamily Grylloidea (Insecta, Orthoptera), mitogenomes of Cacoplistes rogenhoferi and Meloimorpha japonica representing the family Mogoplistidae and three Ornebius species of Phalangopsidae were sequenced. A repeat-containing control region (CR) and 37 genes were present in these mitogenomes. Unusual start codons (TCG, CCG, and CTG) of cox1 and, in Ornebius, a partial stop codon (T) of nad1 followed by a 15-17-bp intergenic spacer were proposed based on transcript information and sequence alignments. The mitogenome-based phylogenetic trees suggest strongly the familial relationships as (((Phalangopsidae + Gryllidae) + Trigonidiidae) + Mogoplistidae). The exclusive occurrence of the trnE-trnS1-trnN rearrangement in Phalangopsidae, Gryllidae, and Trigonidiidae is suggestive of its appearance in the common ancestor of these families after the separation of Mogoplistidae. The trnV transposition in O. bimaculatus and formerly sequenced Trigonidium sjostedti (Trigonidiidae) indicates a potential consequence of parallel evolution. This study offers novel insights into mitogenome evolution, especially gene rearrangements, of Grylloidea.