| Literature DB >> 30171385 |
Brent A Wilkerson1,2, Filippo Artoni1,2, Colby Lea1,2, Kayla Ritchie1,2, Catherine A Ray1,2, Olivia Bermingham-McDonogh3,4.
Abstract
This study examines absolute hair cell numbers in the cristae of C57BL/6J mice and CBA/CaJ mice from weaning to adulthood as well as the dose required for 3,3'-iminodiproprionitrile (IDPN)-injury of the cristae in C57BL/6J mice and CBA/CaJ mice, the two mouse strains most commonly used by inner ear researchers. In cristae of CBA/CaJ and C57BL/6J mice, no loss of hair cells was observed up to 24 weeks. In both strains, dose-dependent loss of hair cells was observed 7 days after IDPN treatment of 2-month-old mice (IC50 = 16.1 mmol/kg in C57BL/6J mice vs. 25.21 mmol/kg in CBA/CaJ mice). Four-month-old C57BL/6J mice exposed to IDPN developed dose-dependent vestibular dysfunction as indicated by increased activity and circling behavior in open field tests and by failure to swim 7 days after treatment. IDPN-hair cell injury in C57BL/6J mice and CBA/CaJ mice represents a fast and predictable experimental model for the study of vestibular degeneration and a platform for the testing of vestibular therapies.Entities:
Keywords: IDPN; cristae; hair cell; mouse; toxicity; vestibular
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Year: 2018 PMID： 30171385 PMCID： PMC6226411 DOI： 10.1007/s10162-018-00687-y
Source DB: PubMed Journal: J Assoc Res Otolaryngol ISSN： 1438-7573