Halla Vidarsdottir1,2,3, Lena Tran4, Björn Nodin3, Karin Jirström3,4, Maria Planck3,5, Johanna S M Mattsson6, Johan Botling6, Patrick Micke6, Per Jönsson7,8, Hans Brunnström3,4. 1. Department of Surgery, Helsingborg Hospital, Helsingborg, Sweden. 2. Department of Clinical Sciences, Lund University, Lund, Sweden. 3. Division of Oncology and Pathology, Lund University, Lund, Sweden. 4. Department of Genetics and Pathology, Laboratory Medicine, Lund, Sweden. 5. Department of Oncology, Skåne University Hospital, Lund, Sweden. 6. Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden. 7. Division of Thoracic Surgery, Lund University, Lund, Sweden. 8. Department of Thoracic Surgery, Skåne University Hospital, Lund, Sweden.
Abstract
OBJECTIVES: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis. METHODS: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases. RESULTS: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas. CONCLUSIONS: TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.
OBJECTIVES: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis. METHODS: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases. RESULTS: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas. CONCLUSIONS: TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.
Authors: Patrick Micke; Johan Botling; Johanna Sofia Margareta Mattsson; Maria Planck; Lena Tran; Halla Vidarsdottir; Björn Nodin; Karin Jirström; Hans Brunnström Journal: Sci Rep Date: 2019-02-04 Impact factor: 4.379
Authors: Kajsa Ericson Lindquist; Inga Gudinaviciene; Nektaria Mylona; Rodrigo Urdar; Maria Lianou; Eva Darai-Ramqvist; Felix Haglund; Mátyás Béndek; Erika Bardoczi; Katalin Dobra; Hans Brunnström Journal: Biomolecules Date: 2021-11-18
Authors: Kajsa Ericson Lindquist; Cristina Ciornei; Sofia Westbom-Fremer; Inga Gudinaviciene; Anna Ehinger; Nektaria Mylona; Rodrigo Urdar; Maria Lianou; Franziska Svensson; Tomas Seidal; Felix Haglund; Katalin Dobra; Mátyás Béndek; Erika Bardóczi; Aneta Szablewska; Marek Witkowski; Maria Ramnefjell; Luis E De Las Casas; Miklos Gulyas; Agnes Hegedus; Patrick Micke; Hans Brunnström Journal: J Clin Pathol Date: 2021-02-05 Impact factor: 4.463