Ivana Lj Nikolic1, Ivan M Savic1, Mirjana M Popsavin2, Srdjan J Rakic3, Tatjana M Mihajilov-Krstev4, Ivan S Ristic5, Suzana P Eric6, Ivana M Savić-Gajic1. 1. Faculty of Technology, University of Nis, Leskovac, Republic of Serbia. 2. Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia. 3. Department of Physics, Faculty of Sciences, University of Novi Sad, Novi Sad, Republic of Serbia. 4. Department of Biology and Ecology, Faculty of Science and Mathematics, University of Nis, Nis, Republic of Serbia. 5. Faculty of Technology, University of Novi Sad, Novi Sad, Republic of Serbia. 6. Faculty of Mining and Geology, University of Belgrade, Belgrade, Republic of Serbia.
Abstract
OBJECTIVES: An inclusion complex of biochanin A (BCA) with (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) was prepared in the ethanol solution to improve its water solubility. METHODS: Using the FTIR, 1 H-NMR, XRD, DSC and SEM methods, the structural characterization of the prepared complex was analysed. KEY FINDINGS: The phase-solubility study has shown that the solubility of BCA was increased twofold in 42% (v/v) ethanol solution after complexation with HP-β-CD. The complex between BCA and HP-β-CD was prepared in the molar ratio of 1 : 1. The antibacterial activity of the inclusion complex was investigated against the various bacteria, fungus and yeast using the microdilution method. The minimal inhibitory concentration values for the analysed strain of bacteria were in the range of 0.84-1.69 mg/cm3 , whereby the prepared inclusion complex exhibited less effect on the reduction of the number of Escherichia coli and Klebsiella pneumoniae species compared to pure BCA. The inclusion complex of BCA was significantly more active against Candida albicans than pure BCA. Biochanin A and its inclusion complex has not expressed the activity against Aspergillus niger. CONCLUSIONS: Based on the obtained results, it can be concluded that the antimicrobial activity of BCA was remained unchanged after complexation.
OBJECTIVES: An inclusion complex of biochanin A (BCA) with (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) was prepared in the ethanol solution to improve its water solubility. METHODS: Using the FTIR, 1 H-NMR, XRD, DSC and SEM methods, the structural characterization of the prepared complex was analysed. KEY FINDINGS: The phase-solubility study has shown that the solubility of BCA was increased twofold in 42% (v/v) ethanol solution after complexation with HP-β-CD. The complex between BCA and HP-β-CD was prepared in the molar ratio of 1 : 1. The antibacterial activity of the inclusion complex was investigated against the various bacteria, fungus and yeast using the microdilution method. The minimal inhibitory concentration values for the analysed strain of bacteria were in the range of 0.84-1.69 mg/cm3 , whereby the prepared inclusion complex exhibited less effect on the reduction of the number of Escherichia coli and Klebsiella pneumoniae species compared to pure BCA. The inclusion complex of BCA was significantly more active against Candida albicans than pure BCA. Biochanin A and its inclusion complex has not expressed the activity against Aspergillus niger. CONCLUSIONS: Based on the obtained results, it can be concluded that the antimicrobial activity of BCA was remained unchanged after complexation.
Authors: Simone Braga Carneiro; Fernanda Ílary Costa Duarte; Luana Heimfarth; Jullyana de Souza Siqueira Quintans; Lucindo José Quintans-Júnior; Valdir Florêncio da Veiga Júnior; Ádley Antonini Neves de Lima Journal: Int J Mol Sci Date: 2019-02-02 Impact factor: 5.923
Authors: Lex Aliko P Balida; Julia Theresa A Regalado; Jade Joshua R Teodosio; Kathryn Ann H Dizon; Zhe Sun; Zhao Qi Zhan; Jenny Marie D Blancaflor; Jan Vincent N Sollesta; Zenith M Villorente; Jonel P Saludes; Doralyn S Dalisay Journal: Metabolites Date: 2022-03-23