Walderez O Dutra1,2, Daniela Faria Barbosa1,3, Paulo Eduardo Alencar de Souza4, Daniel Morgan5, Shelene Poetker6, Luiz Henrique Guimarães7,2, Olívia Bacelar7,2, Kenneth J Gollob8,2, Edgar M Carvalho7,9,2. 1. Laboratory of Cell-Cell Interactions, Department of Morphology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte. 2. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais, Salvador, Bahia, Brazil. 3. Centro Universitário de Formiga, Minas Gerais. 4. Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte. 5. Division of International Medicine and Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, NewYork. 6. Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, NewYork. 7. Immunology Service, Hospital Universitário Prof. Edgar Santos, Universidade Federal da Bahia, Salvador. 8. A.C. Camargo Cancer Center, São Paulo, Universidade Federal da Bahia, Salvador. 9. Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia.
Abstract
Background: Cutaneous leishmaniasis (CL) is characterized by an exaggerated inflammatory response. During pregnancy there is a decreased inflammatory response, and we have shown that pregnant women with CL develop exuberant lesions. Methods: Cytokine production by peripheral blood mononuclear cells and the frequency of cells expressing cytokines in lesions from pregnant and nonpregnant women with CL were evaluated. Results: We observed that CL lesions from pregnant women displayed a more intense cellular infiltrate, associated with an increase in neutrophils and CD4+ cells. While no difference was observed regarding the number of interferon-gamma (IFN-γ)+ cells in lesions from pregnant compared to nonpregnant women with CL, interleukin-10 (IL-10) and IL-4 expression were approximately 3-times higher in lesions in pregnant women. Main sources of IL-4 and IL-10 were CD4+ and CD68+ cells, respectively. Expression of IL-4, but not IFN-γ or IL-10, was positively correlated with the intensity of inflammatory infiltrate in lesions from pregnant women. Conclusions: These results provide evidence of an IL-4-mediated pathology in Leishmania braziliensis-infected pregnant women. These differences in lesion pathogenesis in pregnant and nonpregnant women may open possibilities for new therapies for CL treatment during pregnancy, which are currently lacking.
Background: Cutaneous leishmaniasis (CL) is characterized by an exaggerated inflammatory response. During pregnancy there is a decreased inflammatory response, and we have shown that pregnant women with CL develop exuberant lesions. Methods: Cytokine production by peripheral blood mononuclear cells and the frequency of cells expressing cytokines in lesions from pregnant and nonpregnant women with CL were evaluated. Results: We observed that CL lesions from pregnant women displayed a more intense cellular infiltrate, associated with an increase in neutrophils and CD4+ cells. While no difference was observed regarding the number of interferon-gamma (IFN-γ)+ cells in lesions from pregnant compared to nonpregnant women with CL, interleukin-10 (IL-10) and IL-4 expression were approximately 3-times higher in lesions in pregnant women. Main sources of IL-4 and IL-10 were CD4+ and CD68+ cells, respectively. Expression of IL-4, but not IFN-γ or IL-10, was positively correlated with the intensity of inflammatory infiltrate in lesions from pregnant women. Conclusions: These results provide evidence of an IL-4-mediated pathology in Leishmania braziliensis-infected pregnant women. These differences in lesion pathogenesis in pregnant and nonpregnant women may open possibilities for new therapies for CL treatment during pregnancy, which are currently lacking.
Authors: Alexsandro S Lago; Filipe R Lima; Augusto M Carvalho; Camilla Sampaio; Neuza Lago; Luiz H Guimarães; Jamile Lago; Paulo R L Machado; Lucas P Carvalho; Sérgio Arruda; Edgar M Carvalho Journal: Open Forum Infect Dis Date: 2020-10-19 Impact factor: 3.835
Authors: Neima Briggs; Brian M Wei; Chaarushi Ahuja; Catherine Baker; Carlo Foppiano Palacios; Emily Lee; Niamh O'Grady; Santhi Singanamala; Katelyn Singh; Thilinie D Bandaranayake; Jeffrey M Cohen; William Damsky; Matthew W Davis; Rojelio Mejia; Caroline A Nelson; Jeffrey E Topal; Marwan M Azar Journal: Open Forum Infect Dis Date: 2022-07-22 Impact factor: 4.423