Elske Marra1, Matthijs L Siegenbeek van Heukelom2,3, Annemiek Leeman4, Tim Waterboer5, Chris J L M Meijer6, Peter J F Snijders6, Audrey J King7, Irina Cairo8, Arne van Eeden9, Wilma Brokking9, Pascal van der Weele6,7, Wim Quint4, Jan M Prins3, Henry J C de Vries1,2,10, Maarten F Schim van der Loeff1,10. 1. Department of Infectious Diseases, Public Health Service Amsterdam. 2. Department of Dermatology, University of Amsterdam. 3. Department of Internal Medicine, Academic Medical Center, University of Amsterdam. 4. DDL Diagnostic Laboratory, Rijswijk, the Netherlands. 5. Infections and Cancer Epidemiology, German Cancer Research Center, Heidelberg, Germany. 6. Department of Pathology, Vrije Universiteit-University Medical Center, Amsterdam. 7. Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven. 8. Department of Dermatology, Onze Lieve Vrouwe Gasthuis (OLVG). 9. Department of Internal Medicine, Diagnostisch Centrum (DC) Klinieken. 10. Amsterdam Infection and Immunity Institute, Academic Medical Center, University of Amsterdam, the Netherlands.
Abstract
BACKGROUND: Our objective was to identify virological and serological predictors of anal high-grade squamous intraepithelial lesions (HSIL) in human immunodeficiency virus (HIV)-positive men who have sex with men (MSM). METHODS: HIV-positive MSM were recruited from a longitudinal study during which anal self-swabs and serum were collected at up to 5 bi-annual visits. Swabs were human papillomavirus (HPV) genotyped, and the type-specific HPV viral load in the anal swabs was determined. Serum antibodies to the E6, E7, E1, E2, and L1 proteins of 7 high-risk HPV (hrHPV) types and HPV6 and 11 were analyzed. The participants who had a high-resolution anoscopy after the last study visit were included in the current analysis. Anal HSIL was diagnosed by histopathological examinations of anal biopsies. The causative HPV type of anal HSIL was determined in whole tissue sections (WTS) and by laser capture micro-dissection if more than one HPV-type was found in WTS. Multivariable logistic regression was used to study whether persistent anal HPV infections, HPV viral loads, and seropositivity for HPV were predictors of anal HSIL, either in general or caused by the concordant HPV type. RESULTS: Of 193 HIV-positive MSM, 50 (26%) were diagnosed with anal HSIL. HrHPV persistence in anal swabs was common, varying by hrHPV type between 3-21%. Anal HPV persistence was the only determinant independently associated with anal HSIL, both in general and by concordant, causative HPV type. CONCLUSIONS: Persistent HPV infections were strongly associated with anal HSIL, in general as well as for the concordant HPV type.
BACKGROUND: Our objective was to identify virological and serological predictors of anal high-grade squamous intraepithelial lesions (HSIL) in human immunodeficiency virus (HIV)-positive men who have sex with men (MSM). METHODS:HIV-positive MSM were recruited from a longitudinal study during which anal self-swabs and serum were collected at up to 5 bi-annual visits. Swabs were human papillomavirus (HPV) genotyped, and the type-specific HPV viral load in the anal swabs was determined. Serum antibodies to the E6, E7, E1, E2, and L1 proteins of 7 high-risk HPV (hrHPV) types and HPV6 and 11 were analyzed. The participants who had a high-resolution anoscopy after the last study visit were included in the current analysis. Anal HSIL was diagnosed by histopathological examinations of anal biopsies. The causative HPV type of anal HSIL was determined in whole tissue sections (WTS) and by laser capture micro-dissection if more than one HPV-type was found in WTS. Multivariable logistic regression was used to study whether persistent anal HPV infections, HPV viral loads, and seropositivity for HPV were predictors of anal HSIL, either in general or caused by the concordant HPV type. RESULTS: Of 193 HIV-positive MSM, 50 (26%) were diagnosed with anal HSIL. HrHPV persistence in anal swabs was common, varying by hrHPV type between 3-21%. Anal HPV persistence was the only determinant independently associated with anal HSIL, both in general and by concordant, causative HPV type. CONCLUSIONS: Persistent HPV infections were strongly associated with anal HSIL, in general as well as for the concordant HPV type.
Authors: A Leeman; D Jenkins; E Marra; M van Zummeren; E C Pirog; M M van de Sandt; A van Eeden; M F Schim van der Loeff; J Doorbar; H J C de Vries; F J van Kemenade; C J L M Meijer; W G V Quint Journal: Br J Dermatol Date: 2019-10-02 Impact factor: 9.302
Authors: Carmen Hidalgo-Tenorio; Concepción Gil-Anguita; Miguel Angel López Ruz; Mohamed Omar; Javier López-Hidalgo; Juan Pasquau Journal: PLoS One Date: 2019-10-24 Impact factor: 3.240
Authors: Helen C Stankiewicz Karita; Tim Waterboer; Amalia Magaret; David R Doody; Michael Pawlita; Nicole Brenner; Denise A Galloway; Anna Wald; Margaret M Madeleine Journal: Cancer Epidemiol Biomarkers Prev Date: 2020-09-03 Impact factor: 4.090
Authors: Maria Pujantell; Roger Badia; Iván Galván-Femenía; Edurne Garcia-Vidal; Rafael de Cid; Carmen Alcalde; Antonio Tarrats; Marta Piñol; Francesc Garcia; Ana M Chamorro; Boris Revollo; Sebastian Videla; David Parés; Javier Corral; Cristina Tural; Guillem Sirera; José A Esté; Ester Ballana; Eva Riveira-Muñoz Journal: Sci Rep Date: 2019-12-27 Impact factor: 4.379