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Literature DB >> 3016548

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria.

Abstract

Methotrexate, a folate antagonist, blocks import into mitochondria of mouse dihydrofolate reductase fused to a mitochondrial presequence. Methotrexate does not mask the presequence, but stabilizes the dihydrofolate reductase moiety. It does not inhibit import of the authentic precursor from which the presequence is derived. This suggests that dihydrofolate reductase must at least partly unfold in order to be transported across mitochondrial membranes.

Entities: Chemical Gene Species

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Year: 1986 PMID： 3016548 DOI： 10.1038/322228a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

208 in total

Review 1. Protein unfolding by mitochondria. The Hsp70 import motor.

Authors: A Matouschek; N Pfanner; W Voos
Journal: EMBO Rep Date: 2000-11 Impact factor: 8.807

2. Protein import channel of the outer mitochondrial membrane: a highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small tom proteins, and import receptors.

Authors: C Meisinger; M T Ryan; K Hill; K Model; J H Lim; A Sickmann; H Müller; H E Meyer; R Wagner; N Pfanner
Journal: Mol Cell Biol Date: 2001-04 Impact factor: 4.272

3. In vivo and in vitro interaction of DnaK and a chloroplast transit peptide.

Authors: R A Ivey; B D Bruce
Journal: Cell Stress Chaperones Date: 2000-01 Impact factor: 3.667

4. The mitochondrial Hsp70-dependent import system actively unfolds preproteins and shortens the lag phase of translocation.

Authors: J H Lim; F Martin; B Guiard; N Pfanner; W Voos
Journal: EMBO J Date: 2001-03-01 Impact factor: 11.598

5. Membrane potential-driven protein import into mitochondria. The sorting sequence of cytochrome b(2) modulates the deltapsi-dependence of translocation of the matrix-targeting sequence.

Authors: A Geissler; T Krimmer; U Bömer; B Guiard; J Rassow; N Pfanner
Journal: Mol Biol Cell Date: 2000-11 Impact factor: 4.138

10. Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.

Authors: S T Hwang; G Schatz
Journal: Proc Natl Acad Sci U S A Date: 1989-11 Impact factor: 11.205

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria.

Review 1. Protein unfolding by mitochondria. The Hsp70 import motor.

2. Protein import channel of the outer mitochondrial membrane: a highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small tom proteins, and import receptors.

3. In vivo and in vitro interaction of DnaK and a chloroplast transit peptide.

4. The mitochondrial Hsp70-dependent import system actively unfolds preproteins and shortens the lag phase of translocation.

5. Membrane potential-driven protein import into mitochondria. The sorting sequence of cytochrome b(2) modulates the deltapsi-dependence of translocation of the matrix-targeting sequence.

6. Effect of the protein import machinery at the mitochondrial surface on precursor stability.

7. Yop fusions to tightly folded protein domains and their effects on Yersinia enterocolitica type III secretion.

Review 8. Biophysical studies of recognition sequences for targeting and folding.

9. Biography of Arthur L. Horwich.

10. Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.