Literature DB >> 3016480

Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VII. RNA polymerase is a target for ppGpp.

R E Glass, S T Jones, A Ishihama.   

Abstract

RNA polymerase was isolated from two suppressed rpoB amber strains exhibiting relaxed control over RNA synthesis in vivo. In vitro transcription analysis of these mutant holoenzymes carrying defined substitutions at known sites in the beta subunit clearly shows that single amino acid changes in beta render RNA polymerase resistant to ppGpp. This unambiguously demonstrates that RNA polymerase is indeed the target for ppGpp.

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Year:  1986        PMID： 3016480     DOI： 10.1007/bf00333964

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  21 in total

1.  The mechanism of action of ppGpp on rRNA synthesis in vitro.

Authors:  A J van Ooyen; M Gruber; P Jorgensen
Journal:  Cell       Date:  1976-05       Impact factor: 41.582

2.  Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp.

Authors:  F S Pedersen; E Lund; N O Kjeldgaard
Journal:  Nat New Biol       Date:  1973-05-02

3.  Regulation of membrane phospholipid synthesis by the relA gene: dependence on ppGpp levels.

Authors:  W D Nunn; J E Cronan
Journal:  Biochemistry       Date:  1976-06-15       Impact factor: 3.162

4.  Control of messenger RNA synthesis and decay in Escherichia coli.

Authors:  J D Friesen
Journal:  J Mol Biol       Date:  1966-10       Impact factor: 5.469

5.  Determination of the promoter strength in the mixed transcription system. II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from Escherichia coli.

Authors:  M Kajitani; A Ishihama
Journal:  Nucleic Acids Res       Date:  1983-06-25       Impact factor: 16.971

6.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. III. Analysis of low-level rifampicin-resistant mutants.

Authors:  C M Boothroyd; R M Malet; V Nene; R E Glass
Journal:  Mol Gen Genet       Date:  1983

7.  Heterogeneity of RNA polymerase in Escherichia coli. I. A new holoenzyme containing a new sigma factor.

Authors:  R Fukuda; Y Iwakura; A Ishihama
Journal:  J Mol Biol       Date:  1974-03       Impact factor: 5.469

8.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VI. A redundant region in the beta polypeptide.

Authors:  V Nene; R E Glass
Journal:  Mol Gen Genet       Date:  1984

9.  Promoter selectivity of Escherichia coli RNA polymerase. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons.

Authors:  M Kajitani; A Ishihama
Journal:  J Biol Chem       Date:  1984-02-10       Impact factor: 5.157

10.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. I. The effect of known, single amino acid substitutions in an essential protein.

Authors:  V Nene; R E Glass
Journal:  Mol Gen Genet       Date:  1982
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  18 in total

Review 1.  Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular biosynthetic apparatus with substrates and catalytic components.

Authors:  K F Jensen; S Pedersen
Journal:  Microbiol Rev       Date:  1990-06

2.  The omega subunit of Escherichia coli K-12 RNA polymerase is not required for stringent RNA control in vivo.

Authors:  D Gentry; H Xiao; R Burgess; M Cashel
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Expression of arg genes of Escherichia coli during arginine limitation dependent upon stringent control of translation.

Authors:  M G Williams; P Rogers
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

4.  Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

Authors:  R Landick; A Colwell; J Stewart
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

5.  In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties.

Authors:  G C Rowland; P P Lim; R E Glass
Journal:  Folia Microbiol (Praha)       Date:  1995       Impact factor: 2.099

6.  Multivalent regulation of the nusA operon of Escherichia coli.

Authors:  A Ishihama; A Honda; H Nagasawa-Fujimori; R E Glass; T Maekawa; F Imamoto
Journal:  Mol Gen Genet       Date:  1987-02

7.  Studies in vivo on Escherichia coli RNA polymerase mutants altered in the stringent response.

Authors:  E Baracchini; R Glass; H Bremer
Journal:  Mol Gen Genet       Date:  1988-08

8.  Streptomyces relC mutants with an altered ribosomal protein ST-L11 and genetic analysis of a Streptomyces griseus relC mutant.

Authors:  K Ochi
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

9.  Thermoinducible filamentation in Escherichia coli due to an altered RNA polymerase beta subunit is suppressed by high levels of ppGpp.

Authors:  D Vinella; R D'Ari
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

10.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VIII. Localisation of a region involved in promoter selectivity.

Authors:  R E Glass; S T Jones; V Nene; T Nomura; N Fujita; A Ishihama
Journal:  Mol Gen Genet       Date:  1986-06
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