Kaiming Wu1, Jun Ma2, Yanfeng Zhan3, Kuanzhi Liu4, Ziyin Ye5, Jianhui Chen1, Kaiwu Xu1, Hongli Huang6, Yulong He1. 1. Department of Gastrointestinal Surgery Center, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 2. Department of Thoracic Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 3. Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 4. Department of Anaesthesiology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 5. Department of Pathology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 6. Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
Abstract
BACKGROUND/AIMS: Colon cancer, also known as colorectal cancer (CRC), is one of the most common malignant tumors globally. Although significant advances have been made for developing novel therapeutics, the mechanisms of progression of colorectal cancer are still poorly understood. METHODS: In this study, we identified down-regulation of microRNA-214 (miR-214) as the contributing factor for CRC. Mitochondrial transcription factor A (TFAM) and miR-214 expression in tumor samples from colorectal cancer patients and cancer cell lines were examined by reverse transcription and real-Time PCR (qPCR) or Western Blotting. RESULTS: Our data demonstrated that miR-214 was significantly down-regulated in the tissue samples from CRC patients as well as CRC derived cell lines. TFAM overexpression was also observed in CRC patients and identified as a target for miR-214. Knockdown of TFAM by miR-214 mimics significantly inhibited the proliferation of CRC cell lines. Also, down-regulation of TFAM inhibited nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nuclear translocation and the expression of NF-κB depended genes. CONCLUSION: In conclusion, our data suggested that down-regulation of MiR-214 contributed to the enhanced TFAM expression and decreased proliferation of CRC cells.
BACKGROUND/AIMS: Colon cancer, also known as colorectal cancer (CRC), is one of the most common malignant tumors globally. Although significant advances have been made for developing novel therapeutics, the mechanisms of progression of colorectal cancer are still poorly understood. METHODS: In this study, we identified down-regulation of microRNA-214 (miR-214) as the contributing factor for CRC. Mitochondrial transcription factor A (TFAM) and miR-214 expression in tumor samples from colorectal cancerpatients and cancer cell lines were examined by reverse transcription and real-Time PCR (qPCR) or Western Blotting. RESULTS: Our data demonstrated that miR-214 was significantly down-regulated in the tissue samples from CRC patients as well as CRC derived cell lines. TFAM overexpression was also observed in CRC patients and identified as a target for miR-214. Knockdown of TFAM by miR-214 mimics significantly inhibited the proliferation of CRC cell lines. Also, down-regulation of TFAM inhibited nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nuclear translocation and the expression of NF-κB depended genes. CONCLUSION: In conclusion, our data suggested that down-regulation of MiR-214 contributed to the enhanced TFAM expression and decreased proliferation of CRC cells.
Authors: Veronika Vozáriková; Nina Kunová; Jacob A Bauer; Ján Frankovský; Veronika Kotrasová; Katarína Procházková; Vladimíra Džugasová; Eva Kutejová; Vladimír Pevala; Jozef Nosek; Ľubomír Tomáška Journal: Biomolecules Date: 2020-08-16
Authors: Reyniel Hernández-López; Margalida Torrens-Mas; Daniel G Pons; Maria M Company; Esther Falcó; Teresa Fernández; Javier M Ibarra de la Rosa; Pilar Roca; Jordi Oliver; Jorge Sastre-Serra Journal: Biology (Basel) Date: 2022-02-11