Literature DB >> 30151762

How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique.

Emmanuel Tadjuidje1, Sang-Wook Cha2,3.   

Abstract

We have taken advantage of the well-established oocyte host transfer technique to optimize a method for CRISPR editing of Xenopus that provides an efficient non-mosaic targeted insertion of small DNA fragment through homology-directed repair mechanism.

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Keywords:  CRISPR; F0; HDR; Knock-in; Oocyte; Xenopus

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Year:  2018        PMID: 30151762     DOI: 10.1007/978-1-4939-8784-9_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  1 in total

1.  Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.

Authors:  Jeehyun Jeon; Jung Sun Park; Byungkuk Min; Sun-Ku Chung; Min Kyu Kim; Yong-Kook Kang
Journal:  Front Cell Dev Biol       Date:  2019-11-08
  1 in total

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