Jing Zhao1, Rongfang Li1, Aleksandra Pawlak2, Marta Henklewska2, Angelika Sysak2, Lixin Wen1, Jin-E Yi3, Bożena Obmińska-Mrukowicz4. 1. College of Veterinary Medicine, Hunan Agricultural University, Changsha City, P.R. China. 2. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland. 3. College of Veterinary Medicine, Hunan Agricultural University, Changsha City, P.R. China yijine@gmail.com b.mrukowicz@gmail.com. 4. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland yijine@gmail.com b.mrukowicz@gmail.com.
Abstract
BACKGROUND/AIM: Betulinic acid (BA) and betulin (BT) exhibit a variety of pharmacological properties including anti-cancer, anti-inflammatory and anti-oxidant ones. Canine lymphoma and osteosarcoma have a high mortality rate and need more effective therapeutic approaches. In this study, the anti-proliferative and pro-apoptotic effects of BA and BT were investigated in canine T-cell lymphoma (CL-1), canine B-cell lymphoma (CLBL-1) and canine osteosarcoma (D-17) cell lines. MATERIALS AND METHODS: The cultured cells were treated with several concentrations of BA or BT for 24, 48 and 72 h, and cell proliferation was assessed by the MTT assay. Cell apoptotic rate and cell cycle were analyzed using flow cytometry. RESULTS: Anti-proliferative effect of BT and BA was concentration- and time-dependent. Moreover, BA and BT arrested cell cycle in S phase in CL-1 and D-17 cells, and in G0/G1 phase in CLBL-1 cells. CONCLUSION: Both compounds showed an antitumor activity, and the effects of BA were stronger than that of BT. Copyright
BACKGROUND/AIM: Betulinic acid (BA) and betulin (BT) exhibit a variety of pharmacological properties including anti-cancer, anti-inflammatory and anti-oxidant ones. Caninelymphoma and osteosarcoma have a high mortality rate and need more effective therapeutic approaches. In this study, the anti-proliferative and pro-apoptotic effects of BA and BT were investigated in canineT-cell lymphoma (CL-1), canineB-cell lymphoma (CLBL-1) and canineosteosarcoma (D-17) cell lines. MATERIALS AND METHODS: The cultured cells were treated with several concentrations of BA or BT for 24, 48 and 72 h, and cell proliferation was assessed by the MTT assay. Cell apoptotic rate and cell cycle were analyzed using flow cytometry. RESULTS: Anti-proliferative effect of BT and BA was concentration- and time-dependent. Moreover, BA and BT arrested cell cycle in S phase in CL-1 and D-17 cells, and in G0/G1 phase in CLBL-1 cells. CONCLUSION: Both compounds showed an antitumor activity, and the effects of BA were stronger than that of BT. Copyright
Authors: Alexsander Rodrigues Carvalho Junior; Arthur Lima de Berredo Martins; Brenda da Silva Cutrim; Deivid Martins Santos; Hermerson Sousa Maia; Mari Silma Maia da Silva; Adrielle Zagmignan; Maria Raimunda Chagas Silva; Cristina de Andrade Monteiro; Giselle Maria Skelding Pinheiro Guilhon; Antônio José Cantanhede Filho; Luís Cláudio Nascimento da Silva Journal: Molecules Date: 2019-05-07 Impact factor: 4.411
Authors: Lisa A Weber; Jessica Meißner; Julien Delarocque; Jutta Kalbitz; Karsten Feige; Manfred Kietzmann; Anne Michaelis; Reinhard Paschke; Julia Michael; Barbara Pratscher; Jessika-M V Cavalleri Journal: BMC Vet Res Date: 2020-02-05 Impact factor: 2.741
Authors: Sareh Kamran; Ajantha Sinniah; Mahfoudh A M Abdulghani; Mohammed Abdullah Alshawsh Journal: Cancers (Basel) Date: 2022-02-22 Impact factor: 6.639