| Literature DB >> 3015025 |
P M Thomas, L D Kuykendall, J S Angle.
Abstract
Techniques for in vivo cloning were used with the fast-growing nitrogen-fixing soybean microsymbiont R. fredii USDA 191. Selection for transfer of Tn5 insertions from R. fredii USDA 191 containing the gene-mobilizing plasmid pJB3JI provided recombinants at up to 400 times the background mutation level. These techniques may be useful for future genetic analysis of R. fredii.Entities:
Mesh：
Substances：
Year: 1986 PMID： 3015025 PMCID： PMC203453 DOI： 10.1128/aem.52.1.206-208.1986
Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792