Ashwini P Aithal1, Laxminarayana K Bairy2, Raviraja N Seetharam3, Naveen Kumar1. 1. Department of Anatomy, Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal, Karnataka, India. 2. Department of Pharmacology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah, United Arab Emirates (UAE). 3. Stempeutics Research Pvt. Ltd., Manipal, Karnataka, India.
Abstract
BACKGROUND: To evaluate the haemostatic potential of human bone marrow-derived mesenchymal stromal cells (BM-MSCs) in carbon tetrachloride (CCl4) induced liver cirrhosis in Wistar rats. METHODS: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl4. Rats were randomly divided into 6 groups with ten rats in each group: group 1 (normal control group), group 2 (received only CCl4), group 3 (CCl4 + low dose BM-MSCs), group 4 (CCl4 + high dose BM-MSCs), group 5 (CCl4 + silymarin), group 6 (CCl4 + high dose BM-MSCs + silymarin). Thirty days after the treatment, blood samples were collected for liver enzyme level analysis, prothrombin time test and plasma fibrinogen estimations. The rats were then sacrificed, livers were excised and used for histopathological and scanning electron microscopy (SEM) study. RESULTS: BM-MSCs and the combination treatment of high dose BM-MSCs and silymarin effectively decreased the prothrombin time and increased plasma fibrinogen concentration in rats with CCl4 induced liver cirrhosis. BM-MSCs treatment produces significant anti-fibrotic effect which was supported by the liver enzyme level analysis, histopathology and SEM study. CONCLUSIONS: Results indicate that treatment of BM-MSCs in combination with silymarin had a better haemostatic effect when compared to the administration of BM-MSCs alone.
BACKGROUND: To evaluate the haemostatic potential of human bone marrow-derived mesenchymal stromal cells (BM-MSCs) in carbon tetrachloride (CCl4) induced liver cirrhosis in Wistar rats. METHODS: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl4. Rats were randomly divided into 6 groups with ten rats in each group: group 1 (normal control group), group 2 (received only CCl4), group 3 (CCl4 + low dose BM-MSCs), group 4 (CCl4 + high dose BM-MSCs), group 5 (CCl4 + silymarin), group 6 (CCl4 + high dose BM-MSCs + silymarin). Thirty days after the treatment, blood samples were collected for liver enzyme level analysis, prothrombin time test and plasma fibrinogen estimations. The rats were then sacrificed, livers were excised and used for histopathological and scanning electron microscopy (SEM) study. RESULTS: BM-MSCs and the combination treatment of high dose BM-MSCs and silymarin effectively decreased the prothrombin time and increased plasma fibrinogen concentration in rats with CCl4 induced liver cirrhosis. BM-MSCs treatment produces significant anti-fibrotic effect which was supported by the liver enzyme level analysis, histopathology and SEM study. CONCLUSIONS: Results indicate that treatment of BM-MSCs in combination with silymarin had a better haemostatic effect when compared to the administration of BM-MSCs alone.
Entities:
Keywords:
Mesenchymal stromal cells (MSCs); haemostasis; plasma fibrinogen; prothrombin time
Authors: Scott Maxson; Erasmo A Lopez; Dana Yoo; Alla Danilkovitch-Miagkova; Michelle A Leroux Journal: Stem Cells Transl Med Date: 2012-02 Impact factor: 6.940