INTRODUCTION: Cecropia pachystachya and C. hololeuca are common species in Brazil, popularly used to treat respiratory diseases. Phytochemical studies indicate that their leaves are rich in phenolic compounds, mainly C-glycosilated flavonoids. Orientin and isoorientin are reported in both species, while vitexina and isovitexina were reported in C. pachystachya. In addition, both are rich in chlorogenic acid and have some procyanidins. In some cases, syrups and teas are prepared from leaf blends from different species, which may affect the efficacy and safety of this natural medication. This problem motivated the investigation of the chemical profile of leaves of these species. OBJECTIVE: The phenolic chemical profiles from C. pachystachya and C. hololeuca methanolic extracts were analysed by ultra-high performance liquid chromatography coupled with a diode array detector and mass spectrometry (UPLC-DAD-MS), to investigate possible differences in their metabolite production. MATERIAL AND METHODS: The methanolic extracts of both species were analysed by UPLC-DAD-MS using a C-18 reverse phase column, DAD at 190-400 nm and electrospray ionisation quadruple time-of-flight (ESI-Q-TOF) mass spectrometer. The separation methodology was validated and most of the flavones present in the extracts were quantified. RESULTS: Thirty-seven compounds were tentatively identified, including flavonoids, phenolic acids, flavan-3-ols, condensed tannins (procyanidins) and iridoids, through UV analysis and tandem mass spectrometry (MS/MS) spectra obtained in the negative mode. Chlorogenic acid, orientin and isoorientin were observed as the major constituents in both extracts. CONCLUSIONS: Cecropia pachystachya presented a more diverse chemical profile than C. hololeuca. The methodology developed herein could be an important tool to analyse commercial Cecropia (embaúba) products.
INTRODUCTION:Cecropia pachystachya and C. hololeuca are common species in Brazil, popularly used to treat respiratory diseases. Phytochemical studies indicate that their leaves are rich in phenolic compounds, mainly C-glycosilated flavonoids. Orientin and isoorientin are reported in both species, while vitexina and isovitexina were reported in C. pachystachya. In addition, both are rich in chlorogenic acid and have some procyanidins. In some cases, syrups and teas are prepared from leaf blends from different species, which may affect the efficacy and safety of this natural medication. This problem motivated the investigation of the chemical profile of leaves of these species. OBJECTIVE: The phenolic chemical profiles from C. pachystachya and C. hololeucamethanolic extracts were analysed by ultra-high performance liquid chromatography coupled with a diode array detector and mass spectrometry (UPLC-DAD-MS), to investigate possible differences in their metabolite production. MATERIAL AND METHODS: The methanolic extracts of both species were analysed by UPLC-DAD-MS using a C-18 reverse phase column, DAD at 190-400 nm and electrospray ionisation quadruple time-of-flight (ESI-Q-TOF) mass spectrometer. The separation methodology was validated and most of the flavones present in the extracts were quantified. RESULTS: Thirty-seven compounds were tentatively identified, including flavonoids, phenolic acids, flavan-3-ols, condensed tannins (procyanidins) and iridoids, through UV analysis and tandem mass spectrometry (MS/MS) spectra obtained in the negative mode. Chlorogenic acid, orientin and isoorientin were observed as the major constituents in both extracts. CONCLUSIONS:Cecropia pachystachya presented a more diverse chemical profile than C. hololeuca. The methodology developed herein could be an important tool to analyse commercial Cecropia (embaúba) products.