| Literature DB >> 30141035 |
Chloe Potter1, Didier Hodzic2.
Abstract
The biochemical characterization of proteins most often require their identification by immunoblotting. Whereas SDS-PAGE provides satisfactory results for most proteins, the identification of larger proteins requires alternative methods to ensure their separation and complete transfer onto nitrocellulose membranes. Here, we describe the application of vertical agarose gel electrophoresis to identify large isoforms of nesprin-1 and nesprin-2.Entities:
Keywords: LINC complexes; Nesprin-1 giant; Nesprin-2 giant; Nesprins; SUN proteins; VAGE; Vertical agarose gel electrophoresis
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Year: 2018 PMID： 30141035 DOI： 10.1007/978-1-4939-8691-0_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745