Literature DB >> 3013898

Molecular cloning of cDNA for rat liver gap junction protein.

D L Paul.   

Abstract

An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gt11. The largest cDNA selected contained 1,494 bp and coded for a protein with a calculated molecular mass of 32,007 daltons. Northern blot analysis indicated that brain, kidney, and stomach express an mRNA with similar size and homology to that expressed in liver, but that heart and lens express differently sized, less homologous mRNA.

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Year:  1986        PMID： 3013898      PMCID： PMC2113807          DOI： 10.1083/jcb.103.1.123

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  52 in total

1.  Porin channel triplets merge into single outlets in Escherichia coli outer membranes.

Authors:  A Engel; A Massalski; H Schindler; D L Dorset; J P Rosenbusch
Journal:  Nature       Date:  1985 Oct 17-23       Impact factor: 49.962

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Lens gap junctions: a structural hypothesis for nonregulated low-resistance intercellular pathways.

Authors:  D A Goodenough
Journal:  Invest Ophthalmol Vis Sci       Date:  1979-11       Impact factor: 4.799

4.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

5.  A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.

Authors:  R M Dale; B A McClure; J P Houchins
Journal:  Plasmid       Date:  1985-01       Impact factor: 3.466

6.  Isolation and protein composition of gap junctions from rabbit hearts.

Authors:  C K Manjunath; G E Goings; E Page
Journal:  Biochem J       Date:  1982-07-01       Impact factor: 3.857

7.  Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.

Authors:  D A Goodenough; J S Dick; J E Lyons
Journal:  J Cell Biol       Date:  1980-08       Impact factor: 10.539

8.  Isolation of mouse myocardial gap junctions.

Authors:  R W Kensler; D A Goodenough
Journal:  J Cell Biol       Date:  1980-09       Impact factor: 10.539

9.  Comparative analysis of the major polypeptides from liver gap junctions and lens fiber junctions.

Authors:  E L Hertzberg; D J Anderson; M Friedlander; N B Gilula
Journal:  J Cell Biol       Date:  1982-01       Impact factor: 10.539

10.  Variations in tight and gap junctions in mammalian tissues.

Authors:  D S Friend; N B Gilula
Journal:  J Cell Biol       Date:  1972-06       Impact factor: 10.539
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  169 in total

1.  Different ionic selectivities for connexins 26 and 32 produce rectifying gap junction channels.

Authors:  T M Suchyna; J M Nitsche; M Chilton; A L Harris; R D Veenstra; B J Nicholson
Journal:  Biophys J       Date:  1999-12       Impact factor: 4.033

2.  Targeting motifs and functional parameters governing the assembly of connexins into gap junctions.

Authors:  P E Martin; J Steggles; C Wilson; S Ahmad; W H Evans
Journal:  Biochem J       Date:  2000-07-01       Impact factor: 3.857

3.  Functional coupling between neurons and glia.

Authors:  V Alvarez-Maubecin; F Garcia-Hernandez; J T Williams; E J Van Bockstaele
Journal:  J Neurosci       Date:  2000-06-01       Impact factor: 6.167

4.  Molecular analysis of voltage dependence of heterotypic gap junctions formed by connexins 26 and 32.

Authors:  J B Rubin; V K Verselis; M V Bennett; T A Bargiello
Journal:  Biophys J       Date:  1992-04       Impact factor: 4.033

5.  Mutational analysis of gap junction formation.

Authors:  G Dahl; R Werner; E Levine; C Rabadan-Diehl
Journal:  Biophys J       Date:  1992-04       Impact factor: 4.033

6.  Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70.

Authors:  T W White; R Bruzzone; D A Goodenough; D L Paul
Journal:  Mol Biol Cell       Date:  1992-07       Impact factor: 4.138

7.  A domain substitution procedure and its use to analyze voltage dependence of homotypic gap junctions formed by connexins 26 and 32.

Authors:  J B Rubin; V K Verselis; M V Bennett; T A Bargiello
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

8.  Characteristics of C6 glioma cells overexpressing a gap junction protein.

Authors:  C C Naus; D Zhu; S D Todd; G M Kidder
Journal:  Cell Mol Neurobiol       Date:  1992-04       Impact factor: 5.046

Review 9.  Gap junction channel gating.

Authors:  Feliksas F Bukauskas; Vytas K Verselis
Journal:  Biochim Biophys Acta       Date:  2004-03-23

10.  Differential expression of three gap junction proteins in developing and mature brain tissues.

Authors:  R Dermietzel; O Traub; T K Hwang; E Beyer; M V Bennett; D C Spray; K Willecke
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205
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