Literature DB >> 30138643

The EDTA-based disk-combination tests are unreliable for the detection of MCR-mediated colistin-resistance in Enterobacteriaceae.

Mathieu Clément1, Thomas Büdel2, Odette J Bernasconi1, Luigi Principe3, Vincent Perreten4, Francesco Luzzaro3, Andrea Endimiani5.   

Abstract

We evaluated several EDTA-based combined-disk tests to detect 25 mcr producers among 48 Enterobacteriaceae. Colistin disks plus EDTA (292/584 μg) on MH and CAMH agar were used. Results were positive if with chelator there was an inhibition zone increase ≥3 mm compared to colistin alone. All tests resulted unreliable (sensitivity ≤68%).
Copyright © 2018 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Colistin; Disk; EDTA; Enterobacteriaceae; Phenotype; mcr-1

Mesh:

Substances:

Year:  2018        PMID: 30138643     DOI: 10.1016/j.mimet.2018.08.008

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  2 in total

1.  A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among Enterobacteriales.

Authors:  Drew T Bell; Yehudit Bergman; Abida Q Kazmi; Shawna Lewis; Pranita D Tamma; Patricia J Simner
Journal:  J Clin Microbiol       Date:  2019-04-26       Impact factor: 5.948

2.  Phenotypic Detection of Plasmid-Mediated Colistin Resistance in Enterobacteriaceae.

Authors:  Jose A Di Conza; Gabriel O Gutkind; Edgar Gonzales Escalante; Katherine Yauri Condor
Journal:  J Clin Microbiol       Date:  2020-02-24       Impact factor: 5.948

  2 in total

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