Lea Vacca Michel1, Ravinder Kaur2, Mark Zavorin1, Karin Pryharski2, M Nadeem Khan2,3, Ciara LaClair1, Meghan O'Neil1, Qingfu Xu2, Michael E Pichichero2. 1. 1School of Chemistry and Materials Science, Rochester Institute of Technology, 85 Lomb Memorial Drive, Rochester, NY 14623, USA. 2. 2Rochester General Hospital Research Institute, 1425 Portland Avenue, Rochester, NY 14621, USA. 3. 3Department of Biomedical Sciences, University of North Dakota, 1301 North Columbia Road, Grand Forks, ND 58202, USA.
Abstract
PURPOSE: Nontypeable Haemophilus influenzae (NTHi) is a commensal in the human nasopharynx and the cause of pneumonia, meningitis, sinusitis, acute exacerbations of chronic obstructive pulmonary disease and acute otitis media (AOM). AOM is the most common ailment for which antibiotics are prescribed in the United States. With the emergence of new strains of antibiotic-resistant bacteria, finding an effective and broad coverage vaccine to protect against AOM-causing pathogens has become a priority. Mouse models are a cost-effective and efficient way to help determine vaccine efficacy. Here, we describe an NTHi AOM model in C57BL/6J mice, which also utilizes a mouse-adapted H1N1 influenza virus to mimic human coinfection. METHODOLOGY: We tested our coinfection model using a protein vaccine formulation containing protein D, a well-studied NTHi vaccine candidate that can be found in the 10-valent Streptococcus pneumoniae conjugate vaccine. We verified the usefulness of our mouse model by comparing bacterial loads in the nose and ear between protein D-vaccinated and control mice. RESULTS: While there was no measurable difference in nasal bacterial loads, we did detect significant differences in the bacterial loads of ear washes and ear bullae between vaccinated and control mice. CONCLUSION: The results from this study suggest that our NTHi AOM coinfection model is useful for assessing protein vaccines.
PURPOSE: Nontypeable Haemophilus influenzae (NTHi) is a commensal in the human nasopharynx and the cause of pneumonia, meningitis, sinusitis, acute exacerbations of chronic obstructive pulmonary disease and acute otitis media (AOM). AOM is the most common ailment for which antibiotics are prescribed in the United States. With the emergence of new strains of antibiotic-resistant bacteria, finding an effective and broad coverage vaccine to protect against AOM-causing pathogens has become a priority. Mouse models are a cost-effective and efficient way to help determine vaccine efficacy. Here, we describe an NTHi AOM model in C57BL/6J mice, which also utilizes a mouse-adapted H1N1influenza virus to mimic human coinfection. METHODOLOGY: We tested our coinfection model using a protein vaccine formulation containing protein D, a well-studied NTHi vaccine candidate that can be found in the 10-valent Streptococcus pneumoniae conjugate vaccine. We verified the usefulness of our mouse model by comparing bacterial loads in the nose and ear between protein D-vaccinated and control mice. RESULTS: While there was no measurable difference in nasal bacterial loads, we did detect significant differences in the bacterial loads of ear washes and ear bullae between vaccinated and control mice. CONCLUSION: The results from this study suggest that our NTHi AOM coinfection model is useful for assessing protein vaccines.
Entities:
Keywords:
Protein D; acute otitis media; nontypeable Haemophilus influenzae
Authors: Kelly M Martinovich; Tasmina Rahman; Camilla de Gier; Elke J Seppanen; Tilda Orami; Caitlyn M Granland; Jacinta Francis; Mition Yoannes; Karli J Corscadden; Rebecca Ford; Peter Jacoby; Anita H J van den Biggelaar; Lauren O Bakaletz; Allan W Cripps; Deborah Lehmann; Peter C Richmond; William S Pomat; Lea-Ann S Kirkham; Ruth B Thornton Journal: Front Immunol Date: 2021-08-10 Impact factor: 7.561
Authors: Camilla de Gier; Caitlyn M Granland; Janessa L Pickering; Tony Walls; Mejbah Bhuiyan; Nikki Mills; Peter C Richmond; Emma J Best; Ruth B Thornton; Lea-Ann S Kirkham Journal: Vaccines (Basel) Date: 2019-01-31