Joslaine de Oliveira Nunes1, Rosianne Assis de Sousa Tsujisaki2, Maína de Oliveira Nunes3, Gláucia Moreira Espíndola Lima3, Anamaria Mello Miranda Paniago2, Elenir Rose Jardim Cury Pontes2, Marilene Rodrigues Chang1,4. 1. Programa de Pós-Graduação Stricto Senso em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil. 2. Programa de Pós-Graduação Stricto Senso em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil. 3. Laboratório de Micologia, Hospital Universitário Maria Aparecida Pedrossian, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil. 4. Laboratório de Pesquisa Microbiológica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
Abstract
INTRODUCTION: This study aimed to describe cryptococcal meningitis (CM) cases and the associated demographic, clinical, and microbiological data obtained from cities in the State of Mato Grosso do Sul in the Midwestern region of Brazil. METHODS: The data from 129 patients with laboratory-confirmed CM admitted from 1997 to 2014 were retrospectively reviewed. The molecular types of Cryptococcus neoformans and Cryptococcus gattii isolated from cerebrospinal fluid were analyzed to determine their geographic distribution. RESULTS: The patients had a mean age of 37 years and consisted mostly of men (76.7%). Most of the Cryptococcus isolates were obtained from patients infected with human immunodeficiency virus (HIV) and included 105 (87.5%) and 5 (55.6%) isolates of C. neoformans and C. gattii complexes, respectively. A restriction fragment length polymorphism (RFLP) analysis of URA5 revealed that most of the isolates were C. neoformans molecular type VNI (89.1%), whereas the molecular types VGII (7%) and VNII (3.9%) were observed less frequently. Notably, 65% of the cases with a time from symptom onset to laboratory diagnosis of more than 60 days resulted in fatalities, and sequelae were observed among the patients who survived. CONCLUSIONS: The present study documents the occurrence of neurocryptococcosis, which is mainly caused by C. neoformans VNI, in Mato Grosso do Sul, Brazil, with probable autochthonous cases in the Brazilian Pantanal, the world's largest tropical wetland, and a biome where cryptococcosis has not yet been explored.
INTRODUCTION: This study aimed to describe cryptococcal meningitis (CM) cases and the associated demographic, clinical, and microbiological data obtained from cities in the State of Mato Grosso do Sul in the Midwestern region of Brazil. METHODS: The data from 129 patients with laboratory-confirmed CM admitted from 1997 to 2014 were retrospectively reviewed. The molecular types of Cryptococcus neoformans and Cryptococcus gattii isolated from cerebrospinal fluid were analyzed to determine their geographic distribution. RESULTS: The patients had a mean age of 37 years and consisted mostly of men (76.7%). Most of the Cryptococcus isolates were obtained from patients infected with human immunodeficiency virus (HIV) and included 105 (87.5%) and 5 (55.6%) isolates of C. neoformans and C. gattii complexes, respectively. A restriction fragment length polymorphism (RFLP) analysis of URA5 revealed that most of the isolates were C. neoformans molecular type VNI (89.1%), whereas the molecular types VGII (7%) and VNII (3.9%) were observed less frequently. Notably, 65% of the cases with a time from symptom onset to laboratory diagnosis of more than 60 days resulted in fatalities, and sequelae were observed among the patients who survived. CONCLUSIONS: The present study documents the occurrence of neurocryptococcosis, which is mainly caused by C. neoformans VNI, in Mato Grosso do Sul, Brazil, with probable autochthonous cases in the Brazilian Pantanal, the world's largest tropical wetland, and a biome where cryptococcosis has not yet been explored.
Authors: Aroop Mohanty; Mohit Bhatia; Ankita Kabi; Kuhu Chatterjee; Neelam Kaistha; Balram Ji Omar; Puneet K Gupta; Pratima Gupta Journal: J Family Med Prim Care Date: 2019-06
Authors: Samuel Okurut; David B Meya; Freddie Bwanga; Joseph Olobo; Michael A Eller; Fatim Cham-Jallow; Paul R Bohjanen; Harsh Pratap; Brent E Palmer; Katharine H Hullsiek; Yukari C Manabe; David R Boulware; Edward N Janoff Journal: Infect Immun Date: 2020-02-20 Impact factor: 3.441
Authors: Lidiane de Oliveira; Marcia de Souza Carvalho Melhem; Renata Buccheri; Oscar José Chagas; José Ernesto Vidal; Fredi Alexander Diaz-Quijano Journal: BMC Infect Dis Date: 2022-02-09 Impact factor: 3.090