Comparative Metabolic Responses and Adaptive Strategies of Tea Leaves ( Camellia sinensis) to N2 and CO2 Anaerobic Treatment by a Nontargeted Metabolomics Approach.

It is well-known that anaerobic treatment has been considered as a utility process to accumulate γ-aminobutyric acid (GABA) in tea leaves. In this article, the nonvolatile differential compounds in picked-tea leaves between filled-N2 treatment and filled-CO2 treatment were compared in metabolic profiles and dynamic changes via ultrahigh performance liquid chromatography linked to a hybrid quadrupole orthogonal time-of-flight mass spectrometer (UPLC-Q-TOF-MS). Multivariate analysis and heat map of hierarchical clustering analysis indicated that filled-N2 treatment resulted in a wider range of metabolic perturbation than filled-CO2 treatment, but GABA accumulates faster and more significantly under filled-CO2 treatment than other treatment. The differential metabolites in anaerobic treatment were mainly reflected in the levels of glucose metabolism and amino acid metabolism, and the main differential pathway included the glyoxylate metabolism pathway, galactose metabolism, and phenylalanine metabolism. These metabolomic analyses were also evaluated to illuminate the physiological adaptive strategies of tea adopted to tolerate certain anaerobic stress types.