Gabriella Vácz1, Bálint Major2, Dorottya Gaál1, Laura Petrik1, Dénes Balázs Horváthy1, Weiping Han3, Tünde Holczer4, Melinda Simon1, Jeffrey M Muir5, István Hornyák6, Zsombor Lacza6,7. 1. Institute of Clinical Experimental Research, Semmelweis University, Tűzoltó u. 37-47, Budapest, Hungary, 1094. 2. Polyclinic of the Hospitaller Brothers of St. John of God in Budapest, Orthopaedic Department, Frankel Leo u. 54., Budapest, Hungary, 1023. 3. Bioimaging Consortium, A-STAR, Singapore, Helios, Biopolis Way 11. 4. Department of Laboratory Medicine, Semmelweis University, Nagyvárad t. 4, Budapest, Hungary, 1089. 5. Motion Research, 3-35 Stone Church Rd, Suite 215, Ancaster, Ontario, L9K 3S9 Canada. 6. OrthoSera GmbH, Dr. Karl-Dorrek-Straße 23-29, 3500 Krems an der Donau, Austria. 7. University of Physical Education, Alkotás u. 44, Budapest, Hungary 1123.
Abstract
AIM: Platelet-rich plasma (PRP) and hyperacute serum (HAS) were compared in a novel human model of ex vivo bone damage induced by oxygen-glucose deprivation (OGD). MATERIALS & METHODS: Osteoarthritic subchondral bone pieces were harvested from discarded femoral heads during hip replacement surgery and subjected to transient OGD. RESULTS: Proteome profiling revealed that PRP is more angiopoietic, whereas HAS is more antiangiopoietic in composition. However, treatment of OGD-exposed bone with multiple PRP preparations had no effect on cell counts, whereas HAS restored cell proliferation capacity and rescued viable cell number following OGD. CONCLUSION: A similar pro-proliferation effect was observed with recombinant growth factors, indicating that HAS may be an alternative agent for enhancing the regeneration of damaged bone cells.
AIM: Platelet-rich plasma (PRP) and hyperacute serum (HAS) were compared in a novel human model of ex vivo bone damage induced by oxygen-glucose deprivation (OGD). MATERIALS & METHODS:Osteoarthritic subchondral bone pieces were harvested from discarded femoral heads during hip replacement surgery and subjected to transient OGD. RESULTS: Proteome profiling revealed that PRP is more angiopoietic, whereas HAS is more antiangiopoietic in composition. However, treatment of OGD-exposed bone with multiple PRP preparations had no effect on cell counts, whereas HAS restored cell proliferation capacity and rescued viable cell number following OGD. CONCLUSION: A similar pro-proliferation effect was observed with recombinant growth factors, indicating that HAS may be an alternative agent for enhancing the regeneration of damaged bone cells.
Entities:
Keywords:
blood fractions; bone necrosis; femoral head; growth factors; ischemia
Authors: Dorottya Kardos; Bence Marschall; Melinda Simon; István Hornyák; Adél Hinsenkamp; Olga Kuten; Zsuzsanna Gyevnár; Gábor Erdélyi; Tamás Bárdos; Tamás Mirkó Paukovits; Krisztián Magos; György Béres; Kálmán Szenthe; Ferenc Bánáti; Susan Szathmary; Stefan Nehrer; Zsombor Lacza Journal: Cells Date: 2019-08-03 Impact factor: 6.600