Literature DB >> 3013186

Leukotriene B4 production by stimulated whole blood: comparative studies with isolated polymorphonuclear cells.

P Gresele, J Arnout, M C Coene, H Deckmyn, J Vermylen.   

Abstract

A new method was developed to study leukotriene B4 (LTB4) production by stimulated whole blood. The calcium ionophore A23187 and serum-treated zymosan induced LTB4 production, measured by radioimmunoassay, in a dose- and time-dependent manner. The pattern of LTB4 production by whole blood differed markedly from that observed with isolated, purified polymorphonuclear leukocytes. Higher levels of LTB4 were reached and maintained in whole blood. The system allowed to detect drug effects on LTB4 synthesis in vitro. This new method to study the synthesis of LTB4 takes into account the complex interactions between different cell types which can modulate LTB4 metabolism.

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Year:  1986        PMID： 3013186     DOI： 10.1016/0006-291x(86)91215-5

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Enhanced leukotriene synthesis in leukocytes of atopic and asthmatic subjects.

Authors:  A P Sampson; R U Thomas; J F Costello; P J Piper
Journal:  Br J Clin Pharmacol       Date:  1992-04       Impact factor: 4.335

2.  Selective inhibition of arachidonate 5-lipoxygenase by novel acetohydroxamic acids: biochemical assessment in vitro and ex vivo.

Authors:  J E Tateson; R W Randall; C H Reynolds; W P Jackson; P Bhattacherjee; J A Salmon; L G Garland
Journal:  Br J Pharmacol       Date:  1988-06       Impact factor: 8.739

3.  Orally administered tolfenamic acid inhibits leukotriene synthesis in isolated human peripheral polymorphonuclear leukocytes.

Authors:  E Moilanen; J Alanko; A Juhakoski; H Vapaatalo
Journal:  Agents Actions       Date:  1989-08

4.  Smoking cessation and nicotine substitution modulate eicosanoid synthesis ex vivo in man.

Authors:  A Riutta; V Saareks; I Mucha; J Alanko; M Parviainen; H Vapaatalo
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1995-07       Impact factor: 3.000

5.  Modulation of arachidonic acid metabolism by orally administered morniflumate in man.

Authors:  M Civelli; T Vigano; D Acerbi; P Caruso; M Giossi; S Bongrani; G C Folco
Journal:  Agents Actions       Date:  1991-07

6.  Inhibition of PAF synthesis by stimulated human polymorphonuclear leucocytes with cloricromene, an inhibitor of phospholipase A2 activation.

Authors:  E Ribaldi; A M Mezzasoma; E Francescangeli; M Prosdocimi; G G Nenci; G Goracci; P Gresele
Journal:  Br J Pharmacol       Date:  1996-07       Impact factor: 8.739

7.  Effects of tebufelone (NE-11740), a new anti-inflammatory drug, on arachidonic acid metabolism.

Authors:  S M Weisman; M J Doyle; K R Wehmeyer; B A Hynd; T H Eichhold; R M Clear; C W Coggeshall; D L Kuhlenbeck
Journal:  Agents Actions       Date:  1994-05

8.  Metabolic disposition of leukotriene B4 (LTB4) and oxidation-resistant analogues of LTB4 in conscious rabbits.

Authors:  S Marleau; N Dallaire; P E Poubelle; P Borgeat
Journal:  Br J Pharmacol       Date:  1994-06       Impact factor: 8.739

9.  Spectrophotometric monitoring of lipoxygenase and cyclooxygenase pathway activity using ionophore stimulated whole blood.

Authors:  F J Sweeney; J D Eskra; M J Ernest; T J Carty
Journal:  Agents Actions       Date:  1987-08
  9 in total

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