Miyashaer Hujiahemaiti1, Xiaolin Sun2, Jing Zhou3, Huixin Lv4, Xue Li5, Manlin Qi6, Minghan Chi7, Chunyan Li8, Yanmin Zhou9. 1. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: 397788529@qq.com. 2. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: sxl2673366@126.com. 3. School of Dentistry, Lanzhou University, Lanzhou, China. Electronic address: 329666869@qq.com. 4. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: 125136701@qq.com. 5. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: lixue1166@outlook.com. 6. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: qml1992@126.com. 7. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: jlulynn@126.com. 8. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: jlcyspring@126.com. 9. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China. Electronic address: zhouym62@126.com.
Abstract
BACKGROUND: The width of keratinized mucosa plays an important role in esthetic and functional outcomes of dental implants. Lack of keratinized mucosa may lead to poor oral hygiene and greater soft-tissue recession. This study aimed at assessing the potential of quercetin in promoting human oral keratinocyte (HOK) proliferation and re-epithelialization in vitro. MATERIALS AND METHODS: HOK were detected in the absence or presence of test substances. The Cell Counting Kit-8 was used to assess cell viability and proliferation capacity. Re-epithelization was assessed using a keratinocyte monolayer scratch assay. Cell migration was monitored via Transwell chambers. Porphyromonas gingivalis lipopolysaccharide was used to stimulate keratinocytes for mimicking the inflammatory situation. mRNA expression of inflammatory cytokines (interleukin-1beta, IL-1β and tumor necrosis factor alpha, TNF-α), cell adhesion molecules (Integrin-α6, Integrin-β4), and growth factors (transforming growth factor beta 1,TGF-β1 and transforming growth factor beta 3, TGF-β3) were estimated using RT-qPCR. Protein contents of TGF-β1 and TGF-β3 were investigated by enzyme-linked immunosorbent assay. RESULTS: Multiplex analysis revealed that quercetin enhances HOK proliferation via an upregulation of adhesion molecules (Integrin-α6β4). Additionally, re-epithelialization rate was significantly greater in the presence of quercetin than in the control (P < 0.01). Furthermore, 20 μM of quercetin increases both mRNA and protein levels of TGF-β3 under basal and wound conditions without affecting TGF-β1 production. Expressions of pro-inflammatory cytokines were downregulated by quercetin treatment. CONCLUSION: Quercetin promotes HOKs proliferation and oral re-epithelialization in vitro.
BACKGROUND: The width of keratinized mucosa plays an important role in esthetic and functional outcomes of dental implants. Lack of keratinized mucosa may lead to poor oral hygiene and greater soft-tissue recession. This study aimed at assessing the potential of quercetin in promoting human oral keratinocyte (HOK) proliferation and re-epithelialization in vitro. MATERIALS AND METHODS: HOK were detected in the absence or presence of test substances. The Cell Counting Kit-8 was used to assess cell viability and proliferation capacity. Re-epithelization was assessed using a keratinocyte monolayer scratch assay. Cell migration was monitored via Transwell chambers. Porphyromonas gingivalislipopolysaccharide was used to stimulate keratinocytes for mimicking the inflammatory situation. mRNA expression of inflammatory cytokines (interleukin-1beta, IL-1β and tumornecrosis factor alpha, TNF-α), cell adhesion molecules (Integrin-α6, Integrin-β4), and growth factors (transforming growth factor beta 1,TGF-β1 and transforming growth factor beta 3, TGF-β3) were estimated using RT-qPCR. Protein contents of TGF-β1 and TGF-β3 were investigated by enzyme-linked immunosorbent assay. RESULTS: Multiplex analysis revealed that quercetin enhances HOK proliferation via an upregulation of adhesion molecules (Integrin-α6β4). Additionally, re-epithelialization rate was significantly greater in the presence of quercetin than in the control (P < 0.01). Furthermore, 20 μM of quercetin increases both mRNA and protein levels of TGF-β3 under basal and wound conditions without affecting TGF-β1 production. Expressions of pro-inflammatory cytokines were downregulated by quercetin treatment. CONCLUSION:Quercetin promotes HOKs proliferation and oral re-epithelialization in vitro.
Authors: Tarsizio S Santos; Izabella D D Dos Santos; Rose N Pereira-Filho; Silvana V F Gomes; Isabel B Lima-Verde; Maria N Marques; Juliana C Cardoso; Patricia Severino; Eliana B Souto; Ricardo L C de Albuquerque-Júnior Journal: Curr Issues Mol Biol Date: 2021-06-11 Impact factor: 2.976