Mengyang Du1, Na Yu1, Qiuhong Ding1, Xiaoyue Chen1, Sophia Twum Frempong1, Xushan Cai2, Shihua Bao3. 1. Department of Reproductive Immunology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China. 2. Department of Clinical Laboratory, Maternal and Child Health Hospital of Jiading District, Shanghai 201821, China.. Electronic address: cai139149@126.com. 3. Department of Reproductive Immunology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China. Electronic address: baoshihua@tongji.edu.cn.
Abstract
BACKGROUND: Even though the immune factor is not yet established as a cause of recurrent pregnancy loss (RPL), tons of other studies have shown that a significant proportion of immune abnormalities exist in RPL. METHODS: We conducted a retrospective cohort study with 850 women who were diagnosed with RPL. The percentages of CD3+, CD3+CD4+ and CD3+CD8+T cells of each participant, detected by flow cytometry, were obtained before pregnancy and at 6 weeks of gestation as part of their routine medical examination. RESULTS: Peripheral blood CD3+ T cells prior to pregnancy (at baseline), increased significantly in women who had a miscarriage compared with the subsequent live birth group. Moreover, the percentage of CD3+ and CD3+CD4+T cells during pregnancy increased significantly as compared with the baseline level. After adjusting for potential confounders, the multiple regression equation showed that the CD3+ T cells <67.84% was associated with the risk of miscarriage (OR 1.05, 95% CI, 1.01 to 1.11, p = .04). Additionally, a nonlinear relationship was observed between the percentage of CD3+T cells and the risk of miscarriage. CONCLUSIONS: The risk of miscarriage increased as the percentage of population with CD3+ value below 67.84% has increased, nevertheless, the miscarriage risk did not increase further when the level of CD3+T cells was >67.84%.
BACKGROUND: Even though the immune factor is not yet established as a cause of recurrent pregnancy loss (RPL), tons of other studies have shown that a significant proportion of immune abnormalities exist in RPL. METHODS: We conducted a retrospective cohort study with 850 women who were diagnosed with RPL. The percentages of CD3+, CD3+CD4+ and CD3+CD8+T cells of each participant, detected by flow cytometry, were obtained before pregnancy and at 6 weeks of gestation as part of their routine medical examination. RESULTS: Peripheral blood CD3+ T cells prior to pregnancy (at baseline), increased significantly in women who had a miscarriage compared with the subsequent live birth group. Moreover, the percentage of CD3+ and CD3+CD4+T cells during pregnancy increased significantly as compared with the baseline level. After adjusting for potential confounders, the multiple regression equation showed that the CD3+ T cells <67.84% was associated with the risk of miscarriage (OR 1.05, 95% CI, 1.01 to 1.11, p = .04). Additionally, a nonlinear relationship was observed between the percentage of CD3+T cells and the risk of miscarriage. CONCLUSIONS: The risk of miscarriage increased as the percentage of population with CD3+ value below 67.84% has increased, nevertheless, the miscarriage risk did not increase further when the level of CD3+T cells was >67.84%.
Authors: Songqiang Pang; Yao Song; Jinqiang Yang; Sen Li; Yaqiang He; Gongtang Luo Journal: Evid Based Complement Alternat Med Date: 2021-09-06 Impact factor: 2.629
Authors: Stefanie Dietz; Julian Schwarz; Ana Velic; Irene González-Menéndez; Leticia Quintanilla-Martinez; Nicolas Casadei; Alexander Marmé; Christian F Poets; Christian Gille; Natascha Köstlin-Gille Journal: Front Immunol Date: 2022-01-17 Impact factor: 7.561