Olga De Castro1,2, Mariano Avino3, Antonietta Di Maio4, Bruno Menale5, Marco Guida4,6. 1. Department of Biology, University of Naples Federico II, Via Foria 223, Botanical Garden, 80139, Naples, Italy. olga.decastro@unina.it. 2. DNATech srl, Spin-off Company of the University of Naples Federico II, Botanical Garden, Via Foria 223, 80139, Naples, Italy. olga.decastro@unina.it. 3. Department of Pathology and Laboratory Medicine, Western University, Dental Sciences Building, London, N6A 5C1, Canada. 4. DNATech srl, Spin-off Company of the University of Naples Federico II, Botanical Garden, Via Foria 223, 80139, Naples, Italy. 5. Department of Biology, University of Naples Federico II, Via Foria 223, Botanical Garden, 80139, Naples, Italy. 6. Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy.
Abstract
MAIN CONCLUSION: An interesting AMF colonization microcosm has been detected in the roots of Pancratium maritimum (sea daffodil). Both sequencing techniques (Sanger and NGS) have been used for AMF characterisation, showing a balanced trade-off between pros and cons. By Sanger and next generation sequencing of rRNA nuclear molecular markers (SSU-ITS-LSU and ITS2, respectively), the presence of AMF communities in the roots of P. maritimum was evaluated. Our results shed light on the presence of AMF in sea daffodil and the diversity of assemblages of AMF detected after Sanger sequencing of the SSU-ITS-LSU marker is much higher than that determined following NGS sequencing of ITS2 alone.
MAIN CONCLUSION: An interesting AMF colonization microcosm has been detected in the roots of Pancratium maritimum (sea daffodil). Both sequencing techniques (Sanger and NGS) have been used for AMF characterisation, showing a balanced trade-off between pros and cons. By Sanger and next generation sequencing of rRNA nuclear molecular markers (SSU-ITS-LSU and ITS2, respectively), the presence of AMF communities in the roots of P. maritimum was evaluated. Our results shed light on the presence of AMF in sea daffodil and the diversity of assemblages of AMF detected after Sanger sequencing of the SSU-ITS-LSU marker is much higher than that determined following NGS sequencing of ITS2 alone.
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