| Literature DB >> 30125970 |
Madoka Kuramitsu1, Kazu Okuma1, Makoto Nakashima2, Tomoo Sato3, Daisuke Sasaki4, Hiroo Hasegawa4, Kazumi Umeki5, Ryuji Kubota6, Keiko Sasada7, Rieko Sobata8, Chieko Matsumoto8, Noriaki Kaneko9, Kenta Tezuka1, Sahoko Matsuoka1, Atae Utsunomiya10, Ki-Ryang Koh11, Masao Ogata12, Kenji Ishitsuka13, Mai Taki14, Kisato Nosaka15, Kaoru Uchimaru2,16, Masako Iwanaga17, Yasuko Sagara18, Yoshihisa Yamano3, Akihiko Okayama5, Kiyonori Miura19, Masahiro Satake8, Shigeru Saito20, Toshiki Watanabe2,21, Isao Hamaguchi1.
Abstract
Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08-3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.Entities:
Keywords: human T-cell leukemia virus type 1; proviral load; quantitative PCR; standard
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Year: 2018 PMID： 30125970 DOI： 10.1111/1348-0421.12644
Source DB: PubMed Journal: Microbiol Immunol ISSN： 0385-5600 Impact factor: 1.955