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Literature DB >> 3012554

Purification to apparent homogeneity of a mu-type opioid receptor from rat brain.

Abstract

A mu-opioid-specific receptor was purified to apparent homogeneity from rat brain membranes by 6-succinylmorphine affinity chromatography, gel filtration, wheat germ agglutinin affinity chromatography, and isoelectric focusing. The purified receptor had a molecular weight of 58,000 as determined by NaDodSO4/polyacrylamide gel electrophoresis and was judged to be homogeneous by the following criteria: (i) a single band was detected by autoradiography after NaDodSO4/polyacrylamide gel electrophoresis of 125I-labeled receptor and (ii) the purified preparation had a specific opioid-binding activity of 17,720 pmol/mg of protein, close to the theoretical value. In addition, the Mr 58,000 value agrees closely with that determined by covalently labeling purified receptor with bromoacetyl-[3H]dihydromorphine or with 125I-labeled beta-endorphin and dimethyl suberimidate.

Entities: Chemical Gene Species

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Year: 1986 PMID： 3012554 PMCID： PMC323686 DOI： 10.1073/pnas.83.12.4138

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

1. Multiple opiate receptors. Enkephalins and morphine bind to receptors of different specificity.

Authors: K J Chang; P Cuatrecasas
Journal: J Biol Chem Date: 1979-04-25 Impact factor: 5.157

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. The effects of morphine- and nalorphine- like drugs in the nondependent and morphine-dependent chronic spinal dog.

Authors: W R Martin; C G Eades; J A Thompson; R E Huppler; P E Gilbert
Journal: J Pharmacol Exp Ther Date: 1976-06 Impact factor: 4.030

4. Stereospecific binding of narcotics to brain cerebrosides.

Authors: H H Loh; T M Cho; Y C Wu; E L Way
Journal: Life Sci Date: 1974-06-01 Impact factor: 5.037

5. Stereospecific interaction between narcotic analgesics and a synaptic plasm a membrane fraction of rat cerebral cortex.

Authors: L Terenius
Journal: Acta Pharmacol Toxicol (Copenh) Date: 1973

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Opiate receptor binding: effects of enzymatic treatments.

Authors: G W Pasternak; S H Snyder
Journal: Mol Pharmacol Date: 1974-03 Impact factor: 4.436

8. Stereospecific binding of the potent narcotic analgesic (3H) Etorphine to rat-brain homogenate.

Authors: E J Simon; J M Hiller; I Edelman
Journal: Proc Natl Acad Sci U S A Date: 1973-07 Impact factor: 11.205

9. Experimental autoimmune myasthenia gravis and myasthenia gravis: biochemical and immunochemical aspects.

Authors: J M Lindstrom; V A Lennon; M E Seybold; S Whittingham
Journal: Ann N Y Acad Sci Date: 1976 Impact factor: 5.691

10. 3H-Leu5-enkephalin specific binding to synaptic membrane-comparison with 3H-dihydromorphine and 3H-naloxone.

Authors: P Y Law; H H Loh
Journal: Res Commun Chem Pathol Pharmacol Date: 1978-09

15 in total

1. OPCML gene as a schizophrenia susceptibility locus in Thai population.

Authors: Benjaporn Panichareon; Kazuhiro Nakayama; Wanpen Thurakitwannakarn; Sadahiko Iwamoto; Wasana Sukhumsirichart
Journal: J Mol Neurosci Date: 2011-07-21 Impact factor: 3.444

2. Antinociceptive activity of glycosidic enkephalin analogues.

Authors: R E Rodríguez; F D Rodríguez; M P Sacristán; J L Torres; F Reig; J M García Antón; G Valencia
Journal: Psychopharmacology (Berl) Date: 1990 Impact factor: 4.530

Review 3. The structure and mechanism of neurotransmitter receptors. Implications for the structure and function of the central nervous system.

Authors: P G Strange
Journal: Biochem J Date: 1988-01-15 Impact factor: 3.857

Purification to apparent homogeneity of a mu-type opioid receptor from rat brain.

1. Multiple opiate receptors. Enkephalins and morphine bind to receptors of different specificity.

2. Protein measurement with the Folin phenol reagent.

3. The effects of morphine- and nalorphine- like drugs in the nondependent and morphine-dependent chronic spinal dog.

4. Stereospecific binding of narcotics to brain cerebrosides.

5. Stereospecific interaction between narcotic analgesics and a synaptic plasm a membrane fraction of rat cerebral cortex.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Opiate receptor binding: effects of enzymatic treatments.

8. Stereospecific binding of the potent narcotic analgesic (3H) Etorphine to rat-brain homogenate.

9. Experimental autoimmune myasthenia gravis and myasthenia gravis: biochemical and immunochemical aspects.

10. 3H-Leu5-enkephalin specific binding to synaptic membrane-comparison with 3H-dihydromorphine and 3H-naloxone.

1. OPCML gene as a schizophrenia susceptibility locus in Thai population.

2. Antinociceptive activity of glycosidic enkephalin analogues.

Review 3. The structure and mechanism of neurotransmitter receptors. Implications for the structure and function of the central nervous system.

Review 4. The next frontier in the molecular biology of the opioid system. The opioid receptors.

5. Genetic mapping of opioid binding protein gene(s) to mouse chromosome 9.

6. Postnatal expression profile of OBCAM implies its involvement in visual cortex development and plasticity.

7. Morphine enhances the phosphorylation of a 58 kDa protein in mouse brain membranes.

8. Purification to homogeneity of an active opioid receptor from rat brain by affinity chromatography.

9. Opioid-binding protein (OBCAM) is rich in beta-sheets.

10. Regulation of an opioid-binding protein in NG108-15 cells parallels regulation of delta-opioid receptors.