Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 There exists a distinct stage during mammalian DNA synthesis immediately after joining of replication intermediates.

Literature DB >> 3012484

There exists a distinct stage during mammalian DNA synthesis immediately after joining of replication intermediates.

Abstract

We describe an approach, using alkaline cell lysis and digestion with nuclease S1, which permits to distinguish between newly ligated DNA and the DNA of mature chromatin. When cells with steady-state labelled DNA (mature DNA) are analyzed, the results show labelled "nucleosomal-sized" DNA. However, when DNA of cells pulse-labelled with thymidine for 45 seconds is examined one can detect only large DNA. The newly ligated DNA is not reduced to "nucleosomal-sized" DNA by nuclease S1. When the large DNA is denatured in formamide one can detect 10 kb DNA fragments. Furthermore in pulse-chase experiments there appear, after formamide-treatment, increasing amounts of "nucleosomal-sized" DNA with a parallel decrease in the amount of 10 kb DNA fragments. Hence the newly ligated, large, DNA differs from mature DNA and represents a distinct stage during DNA replication.

Entities: Chemical Species

Mesh：

Substances：

Year: 1986 PMID： 3012484 PMCID： PMC339822 DOI： 10.1093/nar/14.9.3883

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.

Authors: T E Shenk; C Rhodes; P W Rigby; P Berg
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

2. The rate of strand separation in alkali of DNA of irradiated mammalian cells.

Authors: B Rydberg
Journal: Radiat Res Date: 1975-02 Impact factor: 2.841

3. Distribution of histones in alkali-denatured chromatin studied by isopycnic centrifugation in alkaline metrizamide density gradients.

Authors: G Russev; R Tsanev
Journal: Nucleic Acids Res Date: 1976-03 Impact factor: 16.971

There exists a distinct stage during mammalian DNA synthesis immediately after joining of replication intermediates.

1. Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.

2. The rate of strand separation in alkali of DNA of irradiated mammalian cells.

3. Distribution of histones in alkali-denatured chromatin studied by isopycnic centrifugation in alkaline metrizamide density gradients.

4. Isolation and characterisation of a yeast chromosomal replicator.

5. Electron microscopic analysis of chromatin replication in the cellular blastoderm Drosophila melanogaster embryo.

6. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.

7. Dispersive segregation of nucleosomes during replication of simian virus 40 chromosomes.

8. S1 nuclease does not cleave DNA at single-base mis-matches.

Review 9. Replication of eukaryotic chromosomes: a close-up of the replication fork.

10. Eucaryotic DNA: organization of the genome for replication.

1. Conversion of post-elongation stage DNA to mature DNA occurs even if movement of the replication fork has stopped.