Receptors on individual neurones.

Membrane potential or ionic conductance of neurones of the mammalian central or peripheral nervous system maintained in vitro can be measured over periods of several hours. Drugs or transmitters which change potential or conductance can be applied repeatedly under equilibrium conditions, and pharmacological null methods used to characterize the receptors with which they interact. The method offers an advantage over ligand binding studies on nervous tissue because both agonist and antagonist affinities can be estimated on individual functioning cells. The results to date suggest the hypothesis that a given receptor subtype is always associated with the same change in ion conductance, and the corollary that distinct ion conductances affected by the same transmitter result from interactions with different receptor subtypes.