Fikriye Polat1, Meral Yilmaz2, Songul Budak Diler3. 1. Department of Mathematics and Science Education, Faculty of Education, Kocaeli University, Kocaeli 4138, Turkey. fikriyepolat@kocaeli.edu.tr. 2. Department of Research Centre, Faculty of Medicine, Cumhuriyet University, Sivas 58140, Turkey. 3. Department of Biotechnology, Faculty of Science and Letters, Omer Halis Demir University, Nigde 51240, Turkey.
Abstract
PURPOSE: Researchers reported that, MYNN rs10936599 polymorphism is in strong or moderate linkage disequilibrium with SNPs within the 3q26.2 chromosomal regions that also include the TERC gene. In addition, it has been reported that MYNN rs10936599 had a strong cumulative association with bladder cancer risk, and TERC gene suppresses cell growth in bladder cancer cell lines. Therefore, we aimed to determine whether polymorphisms of MYNN rs10936599 and TERC rs2293607 play any roles for bladder cancer in the Turkish population in this study. MATERIALS AND METHODS: In this case-control study, 70 patients and 150 controls were investigated. Genotyping analysis was performed by polymerase chain reaction, restriction fragment length polymorphism and DNA sequencing techniques. RESULTS: Genotype distribution between study groups for MYNN rs10936599 SNP was significantly different (P = .001); although there was no difference in genotype distribution for TERC rs2293607 SNP. In addition, patients with CT genotype and CT+TT genotype combination of MYNN SNP have a decreased risk for bladder cancer. Two times increased risk ratio on development of bladder cancer was obtained for CC genotype of the SNP (P = .001). Besides, it was found that genotype combination of GG+AG/CC versus AA/CC genotypes (TERC/MYNN)showed stronger correlation. We observed that statistically significant relationship between the C-G haplotypes of two polymorphisms and bladder cancer risk (P = .0001). CONCLUSION: At the end of the study, we suggested that there may exist an association between a combination of MYNN rs10936599 and TERC rs2293607 polymorphisms and development of bladder cancer in Turkish population.
PURPOSE: Researchers reported that, MYNNrs10936599 polymorphism is in strong or moderate linkage disequilibrium with SNPs within the 3q26.2 chromosomal regions that also include the TERC gene. In addition, it has been reported that MYNNrs10936599 had a strong cumulative association with bladder cancer risk, and TERC gene suppresses cell growth in bladder cancer cell lines. Therefore, we aimed to determine whether polymorphisms of MYNNrs10936599 and TERCrs2293607 play any roles for bladder cancer in the Turkish population in this study. MATERIALS AND METHODS: In this case-control study, 70 patients and 150 controls were investigated. Genotyping analysis was performed by polymerase chain reaction, restriction fragment length polymorphism and DNA sequencing techniques. RESULTS: Genotype distribution between study groups for MYNNrs10936599 SNP was significantly different (P = .001); although there was no difference in genotype distribution for TERCrs2293607 SNP. In addition, patients with CT genotype and CT+TT genotype combination of MYNN SNP have a decreased risk for bladder cancer. Two times increased risk ratio on development of bladder cancer was obtained for CC genotype of the SNP (P = .001). Besides, it was found that genotype combination of GG+AG/CC versus AA/CC genotypes (TERC/MYNN)showed stronger correlation. We observed that statistically significant relationship between the C-G haplotypes of two polymorphisms and bladder cancer risk (P = .0001). CONCLUSION: At the end of the study, we suggested that there may exist an association between a combination of MYNNrs10936599 and TERCrs2293607 polymorphisms and development of bladder cancer in Turkish population.