Yong Zhang1, Sanxiu Huang2, Yu Guo3, Lingfeng Li4. 1. Department of Obstetrics and Gynecology, Hanchuan city people's Hospital, 431600, China. Electronic address: zhangyongmedc@163.com. 2. Department of Obstetrics and Gynecology, Hanchuan city people's Hospital, 431600, China. Electronic address: huangsanxiumedhb@163.com. 3. Yangtze University, 434023, China. Electronic address: guoyuchhd@163.com. 4. Yangtze University, 434023, China. Electronic address: llfengmecc@163.com.
Abstract
BACKGROUND: Dysregulation of miRNAs is critical for chemosensitivity to platinum-based agents in ovarian cancer (OC) which is the most aggressive gynecological cancer. However, the underlying mechanisms of miRNA-regulated platinum resistance in ovarian cancer remain unclear. In this study, we intended to investigate the effect of miR-1294 on platinum-resistant OC. METHODS: The expression of miR-1294 in OC tissues (n = 30) and cell lines was measured by qRT-PCR. Cell transfection was carried out to establish miR-1294 overexpression or knockdown. MTT and clone formation assays were performed to examine proliferation in OC cells. Additionally, wound healing and tumor invasion assays were used to investigate cell migration and invasion, respectively. Finally, the expression of epithelial-to-mesenchymal transition (EMT)-associated proteins was measured in OC cells by western blot. RESULTS: Our results showed that miR-1294 dysregulation manipulated OC cisplatin resistance through regulating IGF1R. Knockdown of IGF1R decreased SKOVP/DDP cell proliferation, migration, invasion and EMT. Moreover, overexpression of miR-1294 prevented OC cisplatin resistance. CONCLUSION: Our results indicated that epigenetic regulation of IGF1R via miR-1294 was essential for cisplatin resistance in OC and provide a new avenue for OC treatment.
BACKGROUND: Dysregulation of miRNAs is critical for chemosensitivity to platinum-based agents in ovarian cancer (OC) which is the most aggressive gynecological cancer. However, the underlying mechanisms of miRNA-regulated platinum resistance in ovarian cancer remain unclear. In this study, we intended to investigate the effect of miR-1294 on platinum-resistant OC. METHODS: The expression of miR-1294 in OC tissues (n = 30) and cell lines was measured by qRT-PCR. Cell transfection was carried out to establish miR-1294 overexpression or knockdown. MTT and clone formation assays were performed to examine proliferation in OC cells. Additionally, wound healing and tumor invasion assays were used to investigate cell migration and invasion, respectively. Finally, the expression of epithelial-to-mesenchymal transition (EMT)-associated proteins was measured in OC cells by western blot. RESULTS: Our results showed that miR-1294 dysregulation manipulated OC cisplatin resistance through regulating IGF1R. Knockdown of IGF1R decreased SKOVP/DDP cell proliferation, migration, invasion and EMT. Moreover, overexpression of miR-1294 prevented OC cisplatin resistance. CONCLUSION: Our results indicated that epigenetic regulation of IGF1R via miR-1294 was essential for cisplatin resistance in OC and provide a new avenue for OC treatment.