Yutian Tian1, Cengceng Chen2, Shuhan Guo1, Li Zhao1, Yongjian Yan3. 1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, China; Shandong Academy Occupational Health and Occupational Medicine, 18877 Jingshi Road, Jinan 250062, China. 2. Jining Center for Disease Control and Prevention, 26 Yingcui Road, Jining 272000, China. 3. Shandong Academy Occupational Health and Occupational Medicine, 18877 Jingshi Road, Jinan 250062, China. Electronic address: yyj1212015@163.com.
Abstract
OBJECTIVE: We explored methods to establish an animal model of manganese poisoning and evaluate the feasibility of the determination method. METHODS: Twenty-four specific pathogen-free male rats were randomly divided into four groups: control, low-dose (15.0 mg/kg), middle-dose (25.0 mg/kg), and high-dose (50.0 mg/kg). Intraperitoneal injection of MnCl2·H2O was administered every 48 h for three months. Rats were tested for behavior, muscle tension, and with a balance beam experiment at the end of each month. Three months later, the rats were sacrificed and brain tyrosine hydroxylase (TH) expression levels were measured. RESULTS: Rats in each group exhibited changes in behavior, muscle tone, and balance after exposure to manganese, and the scores of each test for the high-dose and middle-dose groups were statistically different from the low-dose and control groups. Finally, a rat model of manganese poisoning was identified with the TH expression less than 30% of the normal value. We find that the modeling success rate of the middle-dose and high-dose groups were 66.67% and 100%, respectively. In addition, there were negative correlations between the three assessment methods such as behavioral tests and TH expression levels. CONCLUSIONS: Intraperitoneal injection of MnCl2·H2O (25 mg/kg) can successfully establish a manganese poisoning rat model with low mortality rate. Muscle tension, balance beam, and behavioral tests can be used as preliminary determination methods for modeling.
OBJECTIVE: We explored methods to establish an animal model of manganesepoisoning and evaluate the feasibility of the determination method. METHODS: Twenty-four specific pathogen-free male rats were randomly divided into four groups: control, low-dose (15.0 mg/kg), middle-dose (25.0 mg/kg), and high-dose (50.0 mg/kg). Intraperitoneal injection of MnCl2·H2O was administered every 48 h for three months. Rats were tested for behavior, muscle tension, and with a balance beam experiment at the end of each month. Three months later, the rats were sacrificed and brain tyrosine hydroxylase (TH) expression levels were measured. RESULTS:Rats in each group exhibited changes in behavior, muscle tone, and balance after exposure to manganese, and the scores of each test for the high-dose and middle-dose groups were statistically different from the low-dose and control groups. Finally, a rat model of manganesepoisoning was identified with the TH expression less than 30% of the normal value. We find that the modeling success rate of the middle-dose and high-dose groups were 66.67% and 100%, respectively. In addition, there were negative correlations between the three assessment methods such as behavioral tests and TH expression levels. CONCLUSIONS: Intraperitoneal injection of MnCl2·H2O (25 mg/kg) can successfully establish a manganesepoisoningrat model with low mortality rate. Muscle tension, balance beam, and behavioral tests can be used as preliminary determination methods for modeling.
Authors: Patricia Morcillo; Hector Cordero; Omamuyovwi M Ijomone; Akinyemi Ayodele; Julia Bornhorst; Leslie Gunther; Frank P Macaluso; Aaron B Bowman; Michael Aschner Journal: Mol Neurobiol Date: 2021-03-05 Impact factor: 5.590