Jana Pourová1, Iveta Najmanová2, Marie Vopršalová1, Thomas Migkos1, Veronika Pilařová3, Lenka Applová1, Lucie Nováková3, Přemysl Mladěnka4. 1. Department of Pharmacology and Toxicology, Charles University, Hradec Králové, Czech Republic. 2. Department of Biological and Medical Sciences, Charles University, Hradec Králové, Czech Republic. 3. Department of Analytical Chemistry, Charles University, Hradec Králové, Czech Republic. 4. Department of Pharmacology and Toxicology, Charles University, Hradec Králové, Czech Republic. Electronic address: mladenkap@faf.cuni.cz.
Abstract
SCOPE: The flavonoid quercetin reduces arterial blood pressure in animals and humans but the mechanisms remains elusive. The aim of this study was to test the activity of flavonoid microbial metabolites, which can participate on the final vasorelaxant effect. METHODS AND RESULTS: Both ex vivo (isolated rat thoracic aorta and mesenteric artery) and in vivo (normotensive and spontaneously hypertensive rats) approaches were used in this study. 4-methylcatechol and 3,4-dihydroxyphenylacetic acid (DHPA) had greater vasorelaxant effects on mesenteric artery than 3-(3-hydroxyphenyl)propionic acid, the previously reported metabolite with vasorelaxant effect. In vivo testing confirmed their blood pressure decreasing effect given both as bolus and slow infusion. Their mechanism at molecular level was different. CONCLUSIONS: This study is the first to show that flavonoid metabolites DHPA and 4-methylcatechol decrease arterial blood pressure and hence a mixture of microbial metabolites formed in the gastrointestinal tract may be responsible for or contribute to the effect of orally ingested quercetin.
SCOPE: The flavonoidquercetin reduces arterial blood pressure in animals and humans but the mechanisms remains elusive. The aim of this study was to test the activity of flavonoid microbial metabolites, which can participate on the final vasorelaxant effect. METHODS AND RESULTS: Both ex vivo (isolated rat thoracic aorta and mesenteric artery) and in vivo (normotensive and spontaneously hypertensiverats) approaches were used in this study. 4-methylcatechol and 3,4-dihydroxyphenylacetic acid (DHPA) had greater vasorelaxant effects on mesenteric artery than 3-(3-hydroxyphenyl)propionic acid, the previously reported metabolite with vasorelaxant effect. In vivo testing confirmed their blood pressure decreasing effect given both as bolus and slow infusion. Their mechanism at molecular level was different. CONCLUSIONS: This study is the first to show that flavonoid metabolites DHPA and 4-methylcatechol decrease arterial blood pressure and hence a mixture of microbial metabolites formed in the gastrointestinal tract may be responsible for or contribute to the effect of orally ingested quercetin.
Authors: Tess De Bruyne; Bieke Steenput; Lynn Roth; Guido R Y De Meyer; Claudia Nunes Dos Santos; Kateřina Valentová; Maija Dambrova; Nina Hermans Journal: Nutrients Date: 2019-03-08 Impact factor: 5.717
Authors: Jana Pourová; Lenka Applová; Kateřina Macáková; Marie Vopršalová; Thomas Migkos; Roger Bentanachs; David Biedermann; Lucie Petrásková; Václav Tvrdý; Marcel Hrubša; Jana Karlíčková; Vladimír Křen; Kateřina Valentová; Přemysl Mladěnka Journal: Nutrients Date: 2019-09-24 Impact factor: 5.717
Authors: Violetta Mohos; Eszter Fliszár-Nyúl; Beáta Lemli; Balázs Zoltán Zsidó; Csaba Hetényi; Přemysl Mladěnka; Pavel Horký; Milan Pour; Miklós Poór Journal: Biomolecules Date: 2020-03-06
Authors: Yue Guo; Wanda J Weber; Dan Yao; Luciano Caixeta; Noah P Zimmerman; Jesse Thompson; Elliot Block; Thomas G Rehberger; Brian A Crooker; Chi Chen Journal: Metabolites Date: 2021-12-24