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Literature DB >> 3011848

Development and application of monoclonal antibodies for specific detection of human enteric adenoviruses.

Abstract

Monoclonal antibodies were prepared against enteric adenovirus by fusing P3-NS1/-Ag4-1 mouse myeloma cells with lymphocytes from BALB/c mice immunized with enteric adenovirus 40 (Ad40) G2297. Of the several putative clones secreting antibodies to adenovirus, five were found to react specifically to the enteric adenovirus. The specificity of two of these monoclones which recognize a single antigen of a molecular size of 17 kilodaltons was evaluated against 78 clinical isolates. One monoclone (5D8/2C2) reacted with both Ad40 and Ad41, and the other monoclone (2H6/C11) recognized Ad40 only in an enzyme-linked immunosorbent assay (ELISA). These ELISA results correlated well with those of the specific neutralization test or DNA restriction endonuclease analysis or both. The use of this rapid ELISA with these monoclones will find applications in the diagnosis of enteric adenovirus and should facilitate the epidemiologic studies of enteric adenovirus gastroenteritis.

Entities: Disease Gene Species

Mesh：

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Year: 1986 PMID： 3011848 PMCID： PMC268733 DOI： 10.1128/jcm.23.5.840-842.1986

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

14 in total

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Journal: Nature Date: 1975-08-07 Impact factor: 49.962

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Authors: L Svensson; G Wadell; I Uhnoo; M Johansson; C H Von Bonsdorff
Journal: J Gen Virol Date: 1983-11 Impact factor: 3.891

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Authors: R K Naz; N J Alexander; M Isahakia; M S Hamilton
Journal: Science Date: 1984-07-20 Impact factor: 47.728

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Journal: Lancet Date: 1981-10-17 Impact factor: 79.321

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Authors: W J Rodriguez; H W Kim; C D Brandt; R H Schwartz; M K Gardner; B Jeffries; R H Parrott; R A Kaslow; J I Smith; H Takiff
Journal: J Pediatr Date: 1985-10 Impact factor: 4.406

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Authors: J C de Jong; R Wigand; A H Kidd; G Wadell; J G Kapsenberg; C J Muzerie; A G Wermenbol; R G Firtzlaff
Journal: J Med Virol Date: 1983 Impact factor: 2.327

9. Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens.

Authors: M Brown; M Petric; P J Middleton
Journal: J Clin Microbiol Date: 1984-09 Impact factor: 5.948

10. Isolation and identification of enteric adenoviruses.

Authors: R Wigand; H G Baumeister; G Maass; J Kühn; H J Hammer
Journal: J Med Virol Date: 1983 Impact factor: 2.327

14 in total

1. DNA hybridization for diagnosis of enteric adenovirus infection from directly spotted human fecal specimens.

Authors: G Hammond; C Hannan; T Yeh; K Fischer; G Mauthe; S E Straus
Journal: J Clin Microbiol Date: 1987-10 Impact factor: 5.948

Review 2. Adenovirus gastroenteritis.

Authors: D J Wood
Journal: Br Med J (Clin Res Ed) Date: 1988-01-23

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Authors: C Niel; S A Gomes; J P Leite; H G Pereira
Journal: J Clin Microbiol Date: 1986-11 Impact factor: 5.948

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Authors: J E Herrmann; D M Perron-Henry; D Stobbs-Walro; N R Blacklow
Journal: Arch Virol Date: 1987 Impact factor: 2.574

5. Fast identification of adenovirus 40/41 in infants with enteritis.

Authors: R Wigand
Journal: Eur J Clin Microbiol Date: 1987-10 Impact factor: 3.267

6. Monoclonal antibody enzyme-linked immunosorbent assay for specific identification and typing of subgroup F adenoviruses.

Authors: N Singh-Naz; W J Rodriguez; A H Kidd; C D Brandt
Journal: J Clin Microbiol Date: 1988-02 Impact factor: 5.948

7. Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes.

Authors: J C de Jong; K Bijlsma; A G Wermenbol; M W Verweij-Uijterwaal; H G van der Avoort; D J Wood; A S Bailey; A D Osterhaus
Journal: J Clin Microbiol Date: 1993-06 Impact factor: 5.948