Literature DB >> 3011506

Solubilization of trehalase from rabbit renal and intestinal brush-border membranes by a phosphatidylinositol-specific phospholipase C.

Y Takesue, K Yokota, Y Nishi, R Taguchi, H Ikezawa.   

Abstract

Trehalase (EC 3.2.1.28) associated with renal and intestinal brush-border membranes was solubilized by highly purified phosphatidylinositol-specific phospholipase C (EC 3.1.4.10) from Bacillus thuringiensis, but not by phosphatidylcholine-hydrolyzing phospholipase C (EC 3.1.4.3) from Clostridium welchii or phospholipase D (EC 3.1.4.4) from cabbage. The solubilized trehalase was not adsorbed on phenyl-Sepharose, indicating that it was hydrophilic. Phosphatidylinositol-specific phospholipase C also converted Triton X-100-solubilized amphipathic trehalase into a hydrophilic form. These results suggest that trehalase is bound to the membrane through a direct and specific interaction with phosphatidylinositol.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3011506     DOI： 10.1016/0014-5793(86)80560-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  13 in total

1.  Pig kidney angiotensin converting enzyme. Purification and characterization of amphipathic and hydrophilic forms of the enzyme establishes C-terminal anchorage to the plasma membrane.

Authors:  N M Hooper; J Keen; D J Pappin; A J Turner
Journal:  Biochem J       Date:  1987-10-01       Impact factor: 3.857

2.  Renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase C.

Authors:  N M Hooper; M G Low; A J Turner
Journal:  Biochem J       Date:  1987-06-01       Impact factor: 3.857

Review 3.  Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchor.

Authors:  M P Lisanti; E Rodriguez-Boulan; A R Saltiel
Journal:  J Membr Biol       Date:  1990-07       Impact factor: 1.843

Review 4.  Modification of proteins with covalent lipids.

Authors:  E N Olson
Journal:  Prog Lipid Res       Date:  1988       Impact factor: 16.195

Review 5.  Intestinal brush border revisited.

Authors:  R Holmes; R W Lobley
Journal:  Gut       Date:  1989-12       Impact factor: 23.059

Review 6.  Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors.

Authors:  M G Low
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857

Review 7.  The regulation of trehalose metabolism in insects.

Authors:  A Becker; P Schlöder; J E Steele; G Wegener
Journal:  Experientia       Date:  1996-05-15

8.  Differences in the glycolipid membrane anchors of bovine and human erythrocyte acetylcholinesterases.

Authors:  W L Roberts; B H Kim; T L Rosenberry
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

9.  Trehalase in the spermatophore from the bean-shaped accessory gland of the male mealworm beetle, Tenebrio molitor: purification, kinetic properties and localization of the enzyme.

Authors:  T Yaginuma; T Mizuno; C Mizuno; M Ikeda; T Wada; K Hattori; O Yamashita; G M Happ
Journal:  J Comp Physiol B       Date:  1996       Impact factor: 2.200

10.  Conjugated bile salts regulate turnover of rat intestinal brush border membrane hydrolases.

Authors:  H Shiozaki; M Yoshioka; S Miura; H Imaeda; A Morita; H Asakura; M Tsuchiya; H Ishii
Journal:  Dig Dis Sci       Date:  1995-06       Impact factor: 3.199
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.