Shuang Bi1, Quan-Zhen Lv1, Tian-Tian Wang1, Beth Burgwyn Fuchs2, Dan-Dan Hu1, Cleo G Anastassopoulou3,4, Athanasios Desalermos4, Maged Muhammed4,5, Chin-Lee Wu6, Yuan-Ying Jiang1, Eleftherios Mylonakis2,3, Yan Wang1,2,4. 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. 2. Division of Infectious Diseases, Rhode Island Hospital, Alpert Medical School of Brown University, RI 02903, USA. 3. Division of Genetics, Cell & Developmental Biology, Department of Biology, University of Patras, Patras, Greece. 4. Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. 5. Division of Infectious Diseases, and Division of Gastroenterology, Boston Children's Hospital. Department of Medicine, Department of Adult Inpatient Medicine, Newton Wellesley Hospital, Harvard Medical School, Boston, MA 02115, USA. 6. Department of Urology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Abstract
AIM: To investigate the role of SDH2 in Candida albicans filamentation and virulence. MATERIALS & METHODS: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. RESULTS: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. CONCLUSION: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. [Formula: see text].
AIM: To investigate the role of SDH2 in Candida albicans filamentation and virulence. MATERIALS & METHODS:Caenorhabditis elegans and mousecandidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. RESULTS: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murinecandidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. CONCLUSION:SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. [Formula: see text].