Literature DB >> 3010947

Removal of haem from lipids extracted from intact erythrocytes with particular reference to polyphosphoinositides.

S Christensen.   

Abstract

With the use of a modified acid version of a current lipid-extraction technique [Bligh & Dyer (1959) Can. J. Biochem. Physiol. 37, 911-917], 92% of phosphatidylinositol 4,5-bisphosphate obtained by means of three sequential extractions from intact human erythrocytes was obtained during the first one. Some 95% of the haem co-extracted with the lipids could then be removed, with a maximal loss of polyphosphoinositides of less than 3%. About 58 nmol of phosphatidylinositol 4,5-bisphosphate was found per ml of erythrocytes.

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Year:  1986        PMID： 3010947      PMCID： PMC1153119          DOI： 10.1042/bj2330921

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  The rapid incorporation of phosphate into mitochondrial lipids.

Authors:  J GARBUS; H F DELUCA; M E LOOMANS; F M STRONG
Journal:  J Biol Chem       Date:  1963-01       Impact factor: 5.157

2.  Calcium-activated hydrolysis of phosphatidyl-myo-inositol 4-phosphate and phosphatidyl-myo-inositol 4,5-bisphosphate in guinea-pig synaptosomes.

Authors:  H D Griffin; J N Hawthorne
Journal:  Biochem J       Date:  1978-11-15       Impact factor: 3.857

3.  Changes in 32 p-labelling of platelet phospholipids in response to ADP.

Authors:  J V Lloyd; E E Nishizawa; J Haldar; J F Mustard
Journal:  Br J Haematol       Date:  1972-11       Impact factor: 6.998

4.  Thin-layer chromatography of the phosphoinositides.

Authors:  F Gonzalez-Sastre; J Folch-Pi
Journal:  J Lipid Res       Date:  1968-07       Impact factor: 5.922

5.  The implication of acid concentration in lipid phosphorus determinations.

Authors:  K S Rhee; L R Dugan
Journal:  Anal Biochem       Date:  1967-04       Impact factor: 3.365

6.  Extraction and purification of polyphosphoinositides.

Authors:  J Schacht
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

7.  Thrombin-induced hydrolysis of phosphatidylinositol in human platelets.

Authors:  R L Bell; P W Majerus
Journal:  J Biol Chem       Date:  1980-03-10       Impact factor: 5.157

8.  Production of 1,2-diacylglycerol and phosphatidate in human erythrocytes treated with calcium ions and ionophore A23187.

Authors:  D Allan; R Watts; R H Michell
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

9.  Effect of neomycin and ionophore A23189 on ATP levels and turnover of polyphosphoinositides in human erythrocytes.

Authors:  V Lang; G Pryhitka; J T Buckley
Journal:  Can J Biochem       Date:  1977-09

10.  Quantification of human platelet inositides and the influence of ionic environment on their incorporation of orthophosphate-32P.

Authors:  P Cohen; M J Broekman; A Verkley; J W Lisman; A Derksen
Journal:  J Clin Invest       Date:  1971-04       Impact factor: 14.808
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  2 in total

1.  Restricted diffusion of integral membrane proteins and polyphosphoinositides leads to their depletion in microvesicles released from human erythrocytes.

Authors:  C Hagelberg; D Allan
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

2.  Interaction between the human immunodeficiency virus type 1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is essential for efficient gag membrane binding.

Authors:  Vineela Chukkapalli; Ian B Hogue; Vitaly Boyko; Wei-Shau Hu; Akira Ono
Journal:  J Virol       Date:  2007-12-19       Impact factor: 5.103
  2 in total

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