| Literature DB >> 3010765 |
Abstract
An assay method for ATP-sulfurylase is described, based on the incorporation of 35SO4(2-) into adenosine-5'-phosphosulfate (and 3-phosphoadenosine-5'-phosphosulfate) and the separation of these compounds by HPLC. Since the enzyme is easily inactivated at temperatures above 20 degrees C during the assay, the reaction time should not exceed 10 min.Entities:
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Year: 1986 PMID： 3010765 DOI： 10.1016/0003-2697(86)90501-4
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365