| Literature DB >> 30102147 |
Alexander S Shashkov1, Johanna J Kenyon2,3, Nikolay P Arbatsky1, Mikhail M Shneider4, Anastasiya V Popova5,6, Yuriy A Knirel1, Ruth M Hall2,7.
Abstract
The KL53 capsular polysaccharide (CPS) gene cluster of Acinetobacter baumannii D23 was sequenced, and includes a single gtr gene encoding the glycosyltransferase Gtr2, and the itrA1 gene for ItrA1 that is known to initiate CPS biosynthesis with d-QuiNAc4NAc. The K53 CPS was isolated and studied by one- and two-dimensional 1H and 13C nuclear magnetic resonance (NMR) spectroscopy before and after O-deacetylation. The disaccharide K unit of the CPS was established as →3)-α-d-GalpNAcA4Ac-(1→3)-β-d-QuipNAc4NAc-(1→, where GalNAcA and QuiNAc4NAc indicate 2-acetamido-2-deoxygalacturonic acid and 2,4-diacetamido-2,4,6-trideoxyglucose, respectively. This established the linkage formed by Gtr2. The degree of 4-O-acetylation of d-GalNAcA by Atr18, encoded at the KL53 locus, is ~55 %.Entities:
Keywords: 2,4-diacetamido-2,4,6-trideoxy-D-glucose; 2-acetamido-2-deoxy-d-galacturonic acid; Acinetobacter baumannii; K locus; KL53; capsular polysaccharide
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Year: 2018 PMID： 30102147 DOI： 10.1099/mic.0.000710
Source DB: PubMed Journal: Microbiology ISSN： 1350-0872 Impact factor: 2.777