Chemical composition of the aqueous humor of bovine eyes dependent on predetermined time upon removal.

The content of several carbohydrates [glucose (alpha and beta), inositol, fructose, galactose] and aminoacids in the aqueous of bovine lenses was measured in dependence on time after globe puncture, using the method of gas liquid chromatography (carbohydrates) and ion exchange chromatography (amino acids). The contents of the investigated compounds show distinct postmortal changes. Aqueous humor which had been obtained immediately after enucleation of the bovine eyes and kept at 37 degrees C for different periods of time (1-8 h) showed for the carbohydrates investigated a decrease in fructose concentration, whereas almost all amino acid concentrations were increased, with the exception of leucine, tyrosine, and arginine, which were decreased.