Francesco Signorelli1, Cyril Pailler-Mattei2, Benjamin Gory3, Pierre Larquet4, Philip Robinson5, Roberto Vargiolu6, Hassan Zahouani6, Paul-Emile Labeyrie7, Jacques Guyotat8, Isabelle Pelissou-Guyotat8, Julien Berthiller9, Francis Turjman7. 1. Division of Neurosurgery, Department of Basic Medical Sciences, Neurosciences and Sense Organs, University "Aldo Moro" of Bari, Bari, Italy; Division of Neurosurgery, Hospices Civils de Lyon, Lyon, France. Electronic address: francesco.signorelli@uniba.it. 2. Tribology and Systems Dynamics Laboratory, UMR-CNRS 5513, "Ecole Centrale de Lyon", Ecully, France; University "Claude Bernard Lyon 1", ISPB-Faculty of Pharmacy, Lyon, France. 3. Department of Diagnostic and Interventional Neuroradiology, University Hospital of Nancy, Nancy, France. 4. University "Claude Bernard Lyon 1", ISPB-Faculty of Pharmacy, Lyon, France. 5. Direction of Clinical Research and Innovation, Hospices Civils de Lyon, Lyon, France. 6. Tribology and Systems Dynamics Laboratory, UMR-CNRS 5513, "Ecole Centrale de Lyon", Ecully, France. 7. Department of Interventional Neuroradiology, Hospices Civils de Lyon, Lyon, France. 8. Division of Neurosurgery, Hospices Civils de Lyon, Lyon, France. 9. Unit of methodological support, "Pôle Information Médicale Evaluation Recherche", Hospices Civils de Lyon, Lyon, France.
Abstract
OBJECTIVE: Aneurysm wall biomechanics are not yet an integral part of aneurysm rupture risk evaluation. We aimed to develop a new technique describing the biomechanical properties of aneurysm wall and correlating them to rupture status. METHODS: Aneurysm wall samples collected during surgery were submitted before and after freezing to tensile tests or as fresh samples to indentation tests. The lateral stiffness or the Young's modulus of the different samples was determined as a function of the mechanical test used. The impact of freezing on biomechanical properties was evaluated. The correlation of clinical and radiologic data with the biomechanical profile of the aneurysm samples was investigated. Two-photon microscopy was used to study collagen fiber organization. RESULTS: Sixteen aneurysm samples (11 unruptured and 5 ruptured) were included. Freezing decreased tissue stiffness. No significant difference was found between ruptured and unruptured aneurysm wall samples regarding demographic characteristics, ethnicity, smoking status, arterial hypertension, site, size and shape of the aneurysm, PHASES score, mechanical profile, or overall Young's modulus. Indentation tests found that the rupture occurred in a restricted area of increased elastic capacity and unruptured areas had increased stiffness. Two-photon microscopy found disruption of the collagen fiber network in rupture zones. CONCLUSIONS: The indentation test of fresh aneurysm wall samples described the heterogeneity of biomechanical properties of the tissue and found increased elastic capacity in the rupture zone and increased stiffness in the remainder of the aneurysm. This study could be a basis for further research aimed at building a biomechanical-based model of aneurysm rupture risk.
OBJECTIVE:Aneurysm wall biomechanics are not yet an integral part of aneurysm rupture risk evaluation. We aimed to develop a new technique describing the biomechanical properties of aneurysm wall and correlating them to rupture status. METHODS:Aneurysm wall samples collected during surgery were submitted before and after freezing to tensile tests or as fresh samples to indentation tests. The lateral stiffness or the Young's modulus of the different samples was determined as a function of the mechanical test used. The impact of freezing on biomechanical properties was evaluated. The correlation of clinical and radiologic data with the biomechanical profile of the aneurysm samples was investigated. Two-photon microscopy was used to study collagen fiber organization. RESULTS: Sixteen aneurysm samples (11 unruptured and 5 ruptured) were included. Freezing decreased tissue stiffness. No significant difference was found between ruptured and unruptured aneurysm wall samples regarding demographic characteristics, ethnicity, smoking status, arterial hypertension, site, size and shape of the aneurysm, PHASES score, mechanical profile, or overall Young's modulus. Indentation tests found that the rupture occurred in a restricted area of increased elastic capacity and unruptured areas had increased stiffness. Two-photon microscopy found disruption of the collagen fiber network in rupture zones. CONCLUSIONS: The indentation test of fresh aneurysm wall samples described the heterogeneity of biomechanical properties of the tissue and found increased elastic capacity in the rupture zone and increased stiffness in the remainder of the aneurysm. This study could be a basis for further research aimed at building a biomechanical-based model of aneurysm rupture risk.
Authors: Joanna Kamińska; Violetta Dymicka-Piekarska; Robert Chrzanowski; Karol Sawicki; Anna J Milewska; Justyna Zińczuk; Marzena Tylicka; Marek Jadeszko; Zenon Mariak; Ewa M Kratz; Joanna Matowicka-Karna; Johannes Kornhuber; Piotr Lewczuk; Olga M Koper-Lenkiewicz Journal: J Inflamm Res Date: 2021-11-23
Authors: Daizo Ishii; Daichi Nakagawa; Mario Zanaty; Jorge A Roa; Sami Al Kasab; Amir Shaban; Joseph S Hudson; Carlos Osorno-Cruz; Stefano Byer; Lauren Allan; James C Torner; Issam A Awad; Timothy J Carroll; Edgar A Samaniego; David M Hasan Journal: J Clin Med Date: 2020-04-01 Impact factor: 4.241